PMID- 9971819
OWN - NLM
STAT- MEDLINE
DCOM- 19990304
LR  - 20240216
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 73
IP  - 3
DP  - 1999 Mar
TI  - Alterations to both the primary and predicted secondary structure of stem-loop 
      IIIc of the hepatitis C virus 1b 5' untranslated region (5'UTR) lead to mutants 
      severely defective in translation which cannot be complemented in trans by the 
      wild-type 5'UTR sequence.
PG  - 2359-64
AB  - Cap-independent translation of the hepatitis C virus (HCV) genomic RNA is 
      mediated by an internal ribosome entry site (IRES) within the 5' untranslated 
      region (5'UTR) of the virus RNA. To investigate the effects of alterations to the 
      primary sequence of the 5'UTR on IRES activity, a series of HCV genotype 1b 
      (HCV-1b) variant IRES elements was generated and cloned into a bicistronic 
      reporter construct. Changes from the prototypic HCV-1b 5'UTR sequence were 
      identified at various locations throughout the 5'UTR. The translation 
      efficiencies of these IRES elements were examined by an in vivo transient 
      expression assay in transfected BHK-21 cells and were found to range from 0.4 to 
      95.8% of the activity of the prototype HCV-1b IRES. Further mutational analysis 
      of the three single-point mutants most severely defective in activity, whose 
      mutations were all located in or near stem-loop IIIc, demonstrated that both the 
      primary sequence and the maintenance of base pairing within this stem structure 
      were critical for HCV IRES function. Complementation studies indicated that 
      defective mutants containing either point mutations or major deletions within the 
      IRES elements could not be complemented in trans by a wild-type IRES.
FAU - Tang, S
AU  - Tang S
AD  - Institute of Virology, University of Glasgow, Glasgow G11 5JR, Scotland, United 
      Kingdom.
FAU - Collier, A J
AU  - Collier AJ
FAU - Elliott, R M
AU  - Elliott RM
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (5' Untranslated Regions)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - 5' Untranslated Regions/*chemistry
MH  - Animals
MH  - Base Sequence
MH  - Cricetinae
MH  - Defective Viruses/genetics
MH  - Genetic Complementation Test
MH  - Hepacivirus/*genetics
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Nucleic Acid Conformation
MH  - *Protein Biosynthesis
MH  - RNA, Viral/*chemistry
MH  - Structure-Activity Relationship
PMC - PMC104481
EDAT- 1999/02/11 00:00
MHDA- 1999/02/11 00:01
PMCR- 1999/03/01
CRDT- 1999/02/11 00:00
PHST- 1999/02/11 00:00 [pubmed]
PHST- 1999/02/11 00:01 [medline]
PHST- 1999/02/11 00:00 [entrez]
PHST- 1999/03/01 00:00 [pmc-release]
AID - 1484 [pii]
AID - 10.1128/JVI.73.3.2359-2364.1999 [doi]
PST - ppublish
SO  - J Virol. 1999 Mar;73(3):2359-64. doi: 10.1128/JVI.73.3.2359-2364.1999.