PMID- 9973384
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20141120
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 162
IP  - 3
DP  - 1999 Feb 1
TI  - Comparison of lung dendritic cells and B cells in stimulating naive 
      antigen-specific T cells.
PG  - 1310-7
AB  - Dendritic cells (DCs) are specialized APCs that are important in priming naive T 
      cells and can be manipulated in vitro and in vivo to enhance immunizations 
      against microorganisms and tumors. A limitation in the development of suitable 
      immunotherapeutic vaccines for the lung is incomplete information on the role of 
      DCs and other potential APCs in the lung in priming naive T cells. In the current 
      study, we analyzed the relative contributions of murine lung DCs and B cells to 
      process and present OVA to naive CD4+ OVA323-339-specific (DO11.10) T cells in 
      vitro. We also examined their expression of MHC class II and accessory molecules 
      before and after maturation in culture. Similar to DCs from other sites, freshly 
      isolated lung DCs can process OVA, spontaneously up-regulate MHC class II and 
      accessory molecules during overnight culture, and stimulate naive T cells in an 
      Ag-specific manner. In contrast, freshly isolated lung B cells were unable to 
      both process and present native OVA. Furthermore, under conditions of limited 
      OVA323-339 peptide exposure, B cells had a significantly diminished capacity to 
      stimulate T cells, and this correlated with a decreased density of both MHC class 
      II and important costimulatory molecules as compared with lung DCs.
FAU - Masten, B J
AU  - Masten BJ
AD  - Department of Pathology, University of New Mexico, Albuquerque, NM 87131, USA. 
      bmasten@salud.unm.edu
FAU - Lipscomb, M F
AU  - Lipscomb MF
LA  - eng
GR  - HL09546/HL/NHLBI NIH HHS/United States
GR  - P50 HL56384/HL/NHLBI NIH HHS/United States
GR  - R01 AI 21951/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (I-Ad antigen)
RN  - 0 (OVA 323-339)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Antigens
MH  - B-Lymphocytes/*immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Histocompatibility Antigens Class II/metabolism
MH  - In Vitro Techniques
MH  - Lung/*cytology/*immunology
MH  - Lymphocyte Activation
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Transgenic
MH  - Ovalbumin/immunology
MH  - Peptide Fragments/immunology
MH  - Receptors, Antigen, T-Cell, alpha-beta/genetics
MH  - T-Lymphocytes/*immunology
EDAT- 1999/02/11 00:00
MHDA- 1999/02/11 00:01
CRDT- 1999/02/11 00:00
PHST- 1999/02/11 00:00 [pubmed]
PHST- 1999/02/11 00:01 [medline]
PHST- 1999/02/11 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1999 Feb 1;162(3):1310-7.