PMID- 9973440
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20171116
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 162
IP  - 3
DP  - 1999 Feb 1
TI  - Direct analysis of viral-specific CD8+ T cells with soluble HLA-A2/Tax11-19 
      tetramer complexes in patients with human T cell lymphotropic virus-associated 
      myelopathy.
PG  - 1765-71
AB  - Human T cell lymphotropic virus-I (HTLV-I)-associated myelopathy is a slowly 
      progressive neurologic disease characterized by inflammatory infiltrates in the 
      central nervous system accompanied by clonal expansion of HTLV-I-reactive CD8+ 
      T-cells. In patients carrying the HLA-A2 allele, the immune response is primarily 
      directed to the Tax11-19 peptide. The frequency, activation state, and TCR usage 
      of HLA-A2/Tax11-19 binding T cells in patients with HTLV-I-associated myelopathy 
      was determined using MHC class I tetramers loaded with the Tax11-19 peptide. 
      Circulating Tax11-19-reactive T cells were found at very high frequencies, 
      approaching 1:10 circulating CD8+ T cells. T cells binding HLA-A2/Tax11-19 
      consisted of heterogeneous populations expressing different chemokine receptors 
      and the IL-2R beta-chain but not the IL-2R alpha-chain. Additionally, 
      Tax11-19-reactive CD8+ T cells used one predominant TCR Vbeta-chain for the 
      recognition of the HLA-A2/Tax11-19 complex. These data provide direct evidence 
      for high frequencies of circulating Tax11-19-reactive CD8+ T cells in patients 
      with HTLV-I-associated myelopathy.
FAU - Bieganowska, K
AU  - Bieganowska K
AD  - Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical 
      School, Boston, MA 02115, USA.
FAU - Hollsberg, P
AU  - Hollsberg P
FAU - Buckle, G J
AU  - Buckle GJ
FAU - Lim, D G
AU  - Lim DG
FAU - Greten, T F
AU  - Greten TF
FAU - Schneck, J
AU  - Schneck J
FAU - Altman, J D
AU  - Altman JD
FAU - Jacobson, S
AU  - Jacobson S
FAU - Ledis, S L
AU  - Ledis SL
FAU - Hanchard, B
AU  - Hanchard B
FAU - Chin, J
AU  - Chin J
FAU - Morgan, O
AU  - Morgan O
FAU - Roth, P A
AU  - Roth PA
FAU - Hafler, D A
AU  - Hafler DA
LA  - eng
GR  - AI42518/AI/NIAID NIH HHS/United States
GR  - P01 AI39671/AI/NIAID NIH HHS/United States
GR  - R01-NS 24247/NS/NINDS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (B7-1 Antigen)
RN  - 0 (CD28 Antigens)
RN  - 0 (Gene Products, tax)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Receptors, Interleukin-2)
SB  - IM
MH  - Adult
MH  - B7-1 Antigen/metabolism
MH  - CD28 Antigens/metabolism
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Female
MH  - Gene Products, tax/*chemistry/*immunology
MH  - HLA-A2 Antigen/*chemistry
MH  - Human T-lymphotropic virus 1/*immunology
MH  - Humans
MH  - Lymphocyte Activation
MH  - Male
MH  - Middle Aged
MH  - Paraparesis, Tropical Spastic/genetics/*immunology
MH  - Peptide Fragments/*chemistry/*immunology
MH  - Phenotype
MH  - Protein Conformation
MH  - Receptors, Antigen, T-Cell, alpha-beta/genetics
MH  - Receptors, Chemokine/metabolism
MH  - Receptors, Interleukin-2/metabolism
MH  - Solubility
EDAT- 1999/02/11 00:00
MHDA- 1999/02/11 00:01
CRDT- 1999/02/11 00:00
PHST- 1999/02/11 00:00 [pubmed]
PHST- 1999/02/11 00:01 [medline]
PHST- 1999/02/11 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1999 Feb 1;162(3):1765-71.