PMID- 9973916
OWN - NLM
STAT- MEDLINE
DCOM- 19990225
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 108
IP  - 1
DP  - 1999 Jan 1
TI  - Abnormal chromosome 8 copy number in stage I to stage IV breast cancer studied by 
      fluorescence in situ hybridization.
PG  - 1-5
AB  - To test the hypothesis that the frequency of abnormal chromosome 8 copy number 
      increases with the severity of the disease as defined by an increase in clinical 
      stage, we conducted a fluorescence in situ hybridization (FISH) study of a sample 
      of 42 breast cancer specimens utilizing a protocol that was optimized by our 
      laboratory. Cytogenetic results, obtained from blinded analyses of archival 
      specimens, demonstrated that the higher clinical stages (i.e., stages III and IV) 
      yield higher frequencies of abnormal chromosome 8 copy number. Specifically, 
      45.45% and 50% of the stage I and stage II cases, respectively, were abnormal, 
      whereas 63.64% and 60% of the stage III and stage IV cases, respectively, were 
      abnormal for chromosome 8 copy number. The overall frequency of abnormal 
      chromosome 8 copy number was 54.76% (23 of 42 tumors studied). When the results 
      of a control probe were taken into account, 34.78% (8 of 23) of the abnormal 
      cases were trisomic, whereas the remaining cases were likely triploid. Thus, the 
      present data not only established that chromosome 8 trisomy is a recurrent 
      finding in breast cancer, but also confirmed a higher frequency of occurrence of 
      abnormal chromosome 8 copy number with the higher clinical stages. Future 
      experiments utilizing additional specimens in this laboratory and from other 
      laboratories are necessary to confirm and extend the findings of the present 
      study.
FAU - Mark, H F
AU  - Mark HF
AD  - Lifespan Academic Medical Center Cytogenetics Laboratory, Providence, USA.
FAU - Taylor, W
AU  - Taylor W
FAU - Brown, S
AU  - Brown S
FAU - Samy, M
AU  - Samy M
FAU - Sun, C L
AU  - Sun CL
FAU - Santoro, K
AU  - Santoro K
FAU - Bland, K I
AU  - Bland KI
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - *Aneuploidy
MH  - Breast Neoplasms/*genetics/*pathology/surgery
MH  - Chromosome Aberrations
MH  - Chromosome Disorders
MH  - *Chromosomes, Human, Pair 8
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Neoplasm Staging
MH  - Observer Variation
MH  - *Trisomy
EDAT- 1999/02/12 00:00
MHDA- 1999/02/12 00:01
CRDT- 1999/02/12 00:00
PHST- 1999/02/12 00:00 [pubmed]
PHST- 1999/02/12 00:01 [medline]
PHST- 1999/02/12 00:00 [entrez]
AID - S0165460898001125 [pii]
AID - 10.1016/s0165-4608(98)00112-5 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Jan 1;108(1):1-5. doi: 
      10.1016/s0165-4608(98)00112-5.