PMID- 9973919
OWN - NLM
STAT- MEDLINE
DCOM- 19990225
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 108
IP  - 1
DP  - 1999 Jan 1
TI  - A combined cytogenetic and molecular approach to diagnosis in a case of 
      desmoplastic small round cell tumor with a complex translocation (11;22;21).
PG  - 19-25
AB  - Desmoplastic small round cell tumor (DSRCT) has recently been described as a 
      discrete tumor entity. It is distinguished from other small round cell tumors by 
      its prominent desmoplastic quality, its preponderance in adolescent males, its 
      almost exclusive intraabdominal location, a multi-immunophenotypic profile, and 
      its aggressive nature. Diagnosis on histology alone is not always unequivocal. A 
      recurrent t(11;22)(p13;q12) translocation has recently been described in this 
      tumor, and a chimeric RNA fusion product formed from the WT1 and EWS genes is 
      detectable by reverse transcriptase-polymerase chain reaction (RT-PCR). We 
      describe the use of a multi-faceted approach using conventional G-banding, 
      fluorescence in situ hybridization (FISH) and RT-PCR to assist the diagnosis of a 
      case of DSRCT with a complex variant t(11;22;21)(p13;q12;q22.1) translocation and 
      demonstrate the value of a combined approach to genetic investigation of solid 
      tumors.
FAU - Roberts, P
AU  - Roberts P
AD  - Regional Cytogenetics Unit, St. James's University Hospital, Leeds, UK.
FAU - Burchill, S A
AU  - Burchill SA
FAU - Beddow, R A
AU  - Beddow RA
FAU - Wheeldon, J
AU  - Wheeldon J
FAU - Cullinane, C
AU  - Cullinane C
FAU - Lewis, I J
AU  - Lewis IJ
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Heterogeneous-Nuclear Ribonucleoproteins)
RN  - 0 (RNA-Binding Protein EWS)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (Transcription Factors)
RN  - 0 (WT1 Proteins)
SB  - IM
MH  - Abdominal Neoplasms/*diagnosis/*genetics/pathology/surgery
MH  - Adolescent
MH  - Base Sequence
MH  - Bone Marrow/pathology
MH  - Carcinoma, Small Cell/*diagnosis/*genetics/pathology/surgery
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 11
MH  - *Chromosomes, Human, Pair 21
MH  - *Chromosomes, Human, Pair 22
MH  - DNA-Binding Proteins/genetics
MH  - Diagnosis, Differential
MH  - Exons
MH  - Genes, Wilms Tumor
MH  - Heterogeneous-Nuclear Ribonucleoproteins
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Molecular Sequence Data
MH  - RNA-Binding Protein EWS
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Ribonucleoproteins/genetics
MH  - Transcription Factors/genetics
MH  - *Translocation, Genetic
MH  - WT1 Proteins
EDAT- 1999/02/12 00:00
MHDA- 1999/02/12 00:01
CRDT- 1999/02/12 00:00
PHST- 1999/02/12 00:00 [pubmed]
PHST- 1999/02/12 00:01 [medline]
PHST- 1999/02/12 00:00 [entrez]
AID - S0165460898001034 [pii]
AID - 10.1016/s0165-4608(98)00103-4 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Jan 1;108(1):19-25. doi: 
      10.1016/s0165-4608(98)00103-4.