PMID- 9973921
OWN - NLM
STAT- MEDLINE
DCOM- 19990225
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 108
IP  - 1
DP  - 1999 Jan 1
TI  - Replication pattern in cancer: asynchronous replication in multiple myeloma and 
      in monoclonal gammopathy.
PG  - 32-7
AB  - In this study we evaluated the replication pattern and cell-cycle dynamics of 
      cells from patients considered to have a premalignant condition (monoclonal 
      gammopathy, or MGUS) and patients with multiple myeloma (MM), as well as healthy 
      controls. We applied the fluorescence in situ hybridization (FISH) technique with 
      the TP53, RB-1 and 21q22 loci on the patient's cells. Asynchrony was determined 
      by the presence of one single and one set of double dots in the same cell. The 
      rate of asynchronic replication was significantly higher in the cells from MM 
      patients, with intermediate value in the cells from MGUS, while the lowest rate 
      was in cells from controls. We suggest that these results may reflect the changes 
      in gene replication and cell-cycle progression that occur in premalignant and 
      malignant cells.
FAU - Amiel, A
AU  - Amiel A
AD  - Genetic Institute, Meir General Hospital, Sapir Medical Center, Kfar-Saba, 
      Israel.
FAU - Kirgner, I
AU  - Kirgner I
FAU - Gaber, E
AU  - Gaber E
FAU - Manor, Y
AU  - Manor Y
FAU - Fejgin, M
AU  - Fejgin M
FAU - Lishner, M
AU  - Lishner M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Aged
MH  - Antineoplastic Combined Chemotherapy Protocols/therapeutic use
MH  - Cell Division
MH  - Cell Nucleus/pathology
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 21
MH  - Female
MH  - *Genes, Retinoblastoma
MH  - *Genes, p53
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/drug therapy/*genetics/*pathology
MH  - Neoplasm Staging
MH  - Paraproteinemias/drug therapy/*genetics/*pathology
MH  - Precancerous Conditions/drug therapy/genetics/pathology
EDAT- 1999/02/12 00:00
MHDA- 1999/02/12 00:01
CRDT- 1999/02/12 00:00
PHST- 1999/02/12 00:00 [pubmed]
PHST- 1999/02/12 00:01 [medline]
PHST- 1999/02/12 00:00 [entrez]
AID - S0165460898001071 [pii]
AID - 10.1016/s0165-4608(98)00107-1 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Jan 1;108(1):32-7. doi: 
      10.1016/s0165-4608(98)00107-1.