PMID- 9973935
OWN - NLM
STAT- MEDLINE
DCOM- 19990223
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 108
IP  - 2
DP  - 1999 Jan 15
TI  - Validation of primed in situ labeling (PRINS) for interphase analysis: 
      comparative studies with conventional fluorescence in situ hybridization and 
      chromosome analyses.
PG  - 100-6
AB  - Primed in situ labeling (PRINS) is a rapidly developing new technology with wide 
      ranging clinical applications. To assess the sensitivity, specificity, and 
      accuracy of PRINS, we carried out a retrospective study on cultured bone marrow 
      cells to detect aneuploidy for chromosomes 7, 8, and 12. The results were then 
      compared to the results of previous fluorescence in situ hybridization (FISH) and 
      chromosome analyses (CA). In patients who showed aneuploidy with CA, both FISH 
      and PRINS confirmed the aneuploidy in interphase cells. FISH and PRINS also 
      showed excellent correlation with conventional cytogenetic analysis for the 
      detection of mosaic aneuploidies. However, both FISH and PRINS showed 
      significantly higher sensitivity in the detection of abnormal clones compared to 
      CA. In 9 of the 17 cases, there were no significant differences in the detection 
      rates between the two methods. Based on our studies, we conclude that PRINS is as 
      sensitive as FISH in most cases for aneuploidy detection; and that PRINS, like 
      FISH, is more sensitive than conventional CA for aneuploidy detection.
FAU - Velagaleti, G V
AU  - Velagaleti GV
AD  - Department of Pediatrics, University of Tennessee Health Sciences Center, 
      Memphis, USA.
FAU - Tharapel, S A
AU  - Tharapel SA
FAU - Tharapel, A T
AU  - Tharapel AT
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Primers)
SB  - IM
MH  - *Aneuploidy
MH  - Base Sequence
MH  - Cells, Cultured
MH  - DNA Primers
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - *Primed In Situ Labeling
MH  - Reproducibility of Results
EDAT- 1999/02/12 00:00
MHDA- 1999/02/12 00:01
CRDT- 1999/02/12 00:00
PHST- 1999/02/12 00:00 [pubmed]
PHST- 1999/02/12 00:01 [medline]
PHST- 1999/02/12 00:00 [entrez]
AID - S0165460898001241 [pii]
AID - 10.1016/s0165-4608(98)00124-1 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Jan 15;108(2):100-6. doi: 
      10.1016/s0165-4608(98)00124-1.