PMID- 9973939
OWN - NLM
STAT- MEDLINE
DCOM- 19990223
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 108
IP  - 2
DP  - 1999 Jan 15
TI  - Trisomy 8 in embryonal rhabdomyosarcoma detected by fluorescence in situ 
      hybridization.
PG  - 127-32
AB  - Embryonal rhabdomyosarcoma is the most common malignant soft-tissue tumor in 
      childhood. Cytogenetic studies of this tumor are rare. In one study trisomy 8 was 
      found to be a primary cytogenetic abnormality. In view of the findings of trisomy 
      8 in a multitude of cancers, we conducted a pilot study to test the hypothesis 
      that a subset of rhabdomyosarcoma also exists with trisomy 8. Accordingly, 
      archival tissues of 12 cases of rhabdomyosarcoma were retrieved and fluorescence 
      in situ hybridization (FISH) using a chromosome 8-specific, alpha-satellite probe 
      was undertaken on formalin-fixed paraffin-embedded tissue sections using the 
      protocol optimized in the Cytogenetics Laboratory at Rhode Island Hospital. The 
      results obtained demonstrated that 6 of 12 tumors showed chromosome 8 trisomy, 
      when a 15% threshold is adopted. In addition, one case was borderline, with 11% 
      of the cells found positive for three fluorescent signals. Future experiments 
      utilizing additional specimens from our centers as well as from other 
      laboratories are needed to confirm and extend the findings of the present study.
FAU - Afify, A
AU  - Afify A
AD  - Department of Pathology, Rhode Island Hospital, Providence 02903, USA.
FAU - Mark, H F
AU  - Mark HF
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Cell Differentiation
MH  - *Chromosomes, Human, Pair 8
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Rhabdomyosarcoma, Embryonal/*genetics/pathology
MH  - *Trisomy
EDAT- 1999/02/12 00:00
MHDA- 1999/02/12 00:01
CRDT- 1999/02/12 00:00
PHST- 1999/02/12 00:00 [pubmed]
PHST- 1999/02/12 00:01 [medline]
PHST- 1999/02/12 00:00 [entrez]
AID - S0165460898001198 [pii]
AID - 10.1016/s0165-4608(98)00119-8 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Jan 15;108(2):127-32. doi: 
      10.1016/s0165-4608(98)00119-8.