PMID- 9973940
OWN - NLM
STAT- MEDLINE
DCOM- 19990223
LR  - 20220409
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 108
IP  - 2
DP  - 1999 Jan 15
TI  - Analysis of sperm chromosome complements before, during, and after chemotherapy.
PG  - 133-6
AB  - Sperm chromosomal abnormalities were assessed in testicular cancer patients 
      before, during, and after BEP (bleomycin, etoposide, cisplatin) chemotherapy 
      (CT). Multicolor fluorescence in situ hybridization (FISH) analysis was employed 
      to detect aneuploidy for chromosomes 1, 12, X, and Y, and diploidy. Sperm samples 
      were cryopreserved and coded before analysis to facilitate "blind" analysis. 
      Complete results at all time points was available for only one patient. A total 
      of 60,400 sperm were analyzed: 20,004 before CT, 20,005 during CT, and 20,391 
      after CT. There was a significant increase in the frequency of 24,XY sperm during 
      (0.33%) and post-CT (0.34%) compared to pre-CT (0.14%). This study suggests that 
      there may be a significantly increased risk of chromosomal abnormalities in sperm 
      of CT patients during and immediately post-CT, similar to that shown in animal 
      models.
FAU - Martin, R H
AU  - Martin RH
AD  - Department of Genetics, Alberta Children's Hospital, University of Calgary, 
      Canada.
FAU - Ernst, S
AU  - Ernst S
FAU - Rademaker, A
AU  - Rademaker A
FAU - Barclay, L
AU  - Barclay L
FAU - Ko, E
AU  - Ko E
FAU - Summers, N
AU  - Summers N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 11056-06-7 (Bleomycin)
RN  - 6PLQ3CP4P3 (Etoposide)
RN  - Q20Q21Q62J (Cisplatin)
RN  - BEP protocol
SB  - IM
MH  - Adult
MH  - Antineoplastic Combined Chemotherapy Protocols/*adverse effects/therapeutic use
MH  - Bleomycin/administration & dosage/adverse effects
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human
MH  - Cisplatin/administration & dosage/adverse effects
MH  - Etoposide/administration & dosage/adverse effects
MH  - Humans
MH  - Male
MH  - Spermatozoa/*drug effects/metabolism
MH  - Testicular Neoplasms/drug therapy/*genetics
EDAT- 1999/02/12 00:00
MHDA- 1999/02/12 00:01
CRDT- 1999/02/12 00:00
PHST- 1999/02/12 00:00 [pubmed]
PHST- 1999/02/12 00:01 [medline]
PHST- 1999/02/12 00:00 [entrez]
AID - S0165460898001253 [pii]
AID - 10.1016/s0165-4608(98)00125-3 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Jan 15;108(2):133-6. doi: 
      10.1016/s0165-4608(98)00125-3.