PMID- 9973954
OWN - NLM
STAT- MEDLINE
DCOM- 19990225
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 109
IP  - 1
DP  - 1999 Feb
TI  - A multimodal approach in the diagnosis of patients with hematopoietic disorders.
PG  - 14-20
AB  - Myelodysplastic syndromes (MDS) are a group of relatively ill-defined 
      hematopoietic disorders in which both qualitative and quantitative defects of the 
      hematopoietic cells cause bone marrow dysfunction. With an incidence estimated to 
      be approximately 1 per 100,000 persons per year, MDS mainly affects the elderly. 
      Myelodysplastic syndromes share many features with acute nonlymphocytic leukemia; 
      in fact, a proportion of patients with MDS eventually develop acute myeloid 
      leukemia. To illustrate a multimodal approach in the diagnosis of patients with 
      hematopoietic disorders, we describe a 66-year-old patient with a question of 
      myelodysplastic syndrome, leukemia, and two translocations involving chromosome 
      10:t(5;10) and t(7;10). These structural rearrangements effectively gave rise to 
      monosomy for part of the long arm of chromosome 5 and for the long arm of 
      chromosome 7. Findings of del(5q) and del(7) in MDS have been reported in the 
      literature. The results of chromosome morphometry, which was conducted to compare 
      the lengths of all relevant chromosome segments, are consistent with the 
      hypothesized chromosomal abnormalities. The investigational technique of 
      fluorescence in situ hybridization (FISH), using both painting and 
      alpha-satellite probes, was used as an adjunct to conventional cytogenetics to 
      further delineate the nature of the chromosome abnormalities observed in the 
      GTG-banded studies. Confirmatory studies utilizing the new technique of spectral 
      karyotyping (SKY) were also carried out. Thus, the multimodal approach of 
      hematopathology, GTG-banding, chromosome morphometry, FISH, and SKY can be very 
      useful for delineating complex cytogenetic cases.
FAU - Mark, H F
AU  - Mark HF
AD  - Memorial Hospital of Rhode Island, Pawtucket, USA.
FAU - Gray, Y
AU  - Gray Y
FAU - Mark, Y
AU  - Mark Y
FAU - Khorsand, J
AU  - Khorsand J
FAU - Sikov, W
AU  - Sikov W
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Bone Marrow/pathology
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 5
MH  - *Chromosomes, Human, Pair 7
MH  - Cytogenetics/methods
MH  - Diagnosis, Differential
MH  - Hematopoiesis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Incidence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*diagnosis/epidemiology/genetics/pathology
EDAT- 1999/02/12 00:00
MHDA- 1999/02/12 00:01
CRDT- 1999/02/12 00:00
PHST- 1999/02/12 00:00 [pubmed]
PHST- 1999/02/12 00:01 [medline]
PHST- 1999/02/12 00:00 [entrez]
AID - S0165460898001423 [pii]
AID - 10.1016/s0165-4608(98)00142-3 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Feb;109(1):14-20. doi: 
      10.1016/s0165-4608(98)00142-3.