PMID- 9987694
OWN - NLM
STAT- MEDLINE
DCOM- 19990421
LR  - 20190515
IS  - 1058-0468 (Print)
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Linking)
VI  - 16
IP  - 1
DP  - 1999 Jan
TI  - Fluorescence in situ hybridization with chromosome paint probes: a novel approach 
      to assess aneuploidy in human sperm nuclei.
PG  - 46-51
AB  - PURPOSE: Fluorescence in situ hybridization (FISH) using whole-chromosome paint 
      probes was performed to evaluate disomy and diploidy frequency for chromosomes 1, 
      18, 19, and 22 in human sperm nuclei. METHODS: Ten subjects of proven fertility 
      and normal spermatic parameters were included in the study. A dual-color FISH 
      method was carried out. RESULTS: A total of 157,896 spermatozoa was scored. The 
      mean frequencies of disomic sperm for chromosomes 1, 18, 19, and 22 were 0.22% 
      (range, 0.19 to 0.28%), 0.24% (range, 0.14 to 0.37%), 0.22% (range, 0.17 to 
      0.30%), and 0.25% (range, 0.21 to 0.29%), respectively. The mean frequency of 
      diploidy was 0.14% (range, 0.09 to 0.18%). No interindividual and 
      interchromosomal variations in the aneuploidy frequency were observed between the 
      different subjects. CONCLUSIONS: FISH with whole-chromosome paint probes provides 
      a novel and efficient approach for disomy assessment in human sperm nuclei.
FAU - Rives, N
AU  - Rives N
AD  - Reproductive Biology Laboratory, Rouen University Hospital, France.
FAU - Wust, S
AU  - Wust S
FAU - David, B
AU  - David B
FAU - Duchesne, V
AU  - Duchesne V
FAU - Joly, G
AU  - Joly G
FAU - Mace, B
AU  - Mace B
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Cell Nucleus/*genetics
MH  - *Chromosome Painting
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Chromosomes, Human, Pair 18/genetics
MH  - Chromosomes, Human, Pair 19/genetics
MH  - Chromosomes, Human, Pair 22/genetics
MH  - Diploidy
MH  - Humans
MH  - Male
MH  - *Spermatozoa
PMC - PMC3468216
EDAT- 1999/02/13 00:00
MHDA- 1999/02/13 00:01
PMCR- 2000/01/01
CRDT- 1999/02/13 00:00
PHST- 1999/02/13 00:00 [pubmed]
PHST- 1999/02/13 00:01 [medline]
PHST- 1999/02/13 00:00 [entrez]
PHST- 2000/01/01 00:00 [pmc-release]
AID - 421832 [pii]
AID - 10.1023/a:1022549713692 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 1999 Jan;16(1):46-51. doi: 10.1023/a:1022549713692.