PMID- 9987901
OWN - NLM
STAT- MEDLINE
DCOM- 19990318
LR  - 20190513
IS  - 0022-1503 (Print)
IS  - 0022-1503 (Linking)
VI  - 90
IP  - 1
DP  - 1999 Jan-Feb
TI  - A comparative approach to physical and linkage mapping of genes on canine 
      chromosomes using gene-associated simple sequence repeat polymorphisms 
      illustrated by studies of dog chromosome 9.
PG  - 39-42
AB  - We describe and illustrate a comparative approach to creating physical and 
      linkage maps of genes on dog chromosomes. The approach is particularly useful in 
      species, like the dog, which have a rudimentary gene map not integrated with 
      microsatellite loci. Human or mouse cDNAs for genes to be mapped are used to 
      isolate cosmid or phage clones from dog genomic libraries. Clones verified to 
      contain the homologous canine gene coding sequences are screened for 
      "gene-associated" simple sequence repeat polymorphisms (SSRPs). The unique 
      sequences flanking the repeats are used to design PCR primers to amplify the 
      repeat and gene-associated SSR length differences that are informative for 
      linkage analysis used in canine pedigrees to study linkage between loci or with 
      diseases. The same canine clones are employed as probes in fluorescence in situ 
      hybridization (FISH) studies to physically map the loci to specific sites on dog 
      chromosomes. This approach creates a combined gene and gene-associated 
      microsatellite anchor locus framework map. In this article we review our recent 
      use of this approach to map a series of genes found on human chromosome 17 
      (HSA17) to two dog chromosomes. Canine chromosome 9 (CFA9) contains 11 loci found 
      on HSA17q, while two genes from HSA17p map to CFA5, demonstrating disruption of 
      HSA17 synteny at the centromere. The order of 11 HSA17q genes on CFA9 was 
      conserved in the dog, but the entire group is inverted with respect to the 
      centromere when compared to human and mouse. Maps created by this approach can be 
      used to advantage for integrating anonymous microsatellites with gene maps, 
      including microsatellites found in genome scans to be linked to canine diseases. 
      This makes it possible to identify the homologous chromosomal region in the human 
      or mouse genome and to make use of this information in formulating hypotheses 
      regarding candidate genes, as has recently been illustrated by other 
      investigators.
FAU - Werner, P
AU  - Werner P
AD  - Center for Comparative Medical Genetics, School of Veterinary Medicine, 
      University of Pennsylvania, Philadelphia 19104-6010, USA.
FAU - Raducha, M G
AU  - Raducha MG
FAU - Prociuk, U
AU  - Prociuk U
FAU - Henthorn, P S
AU  - Henthorn PS
FAU - Patterson, D F
AU  - Patterson DF
LA  - eng
GR  - HL18848/HL/NHLBI NIH HHS/United States
GR  - RR02512/RR/NCRR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - United States
TA  - J Hered
JT  - The Journal of heredity
JID - 0375373
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - Chromosome Banding/veterinary
MH  - *Chromosomes
MH  - Chromosomes, Human, Pair 17
MH  - Dogs/*genetics
MH  - *Genetic Linkage
MH  - Genetic Markers
MH  - Humans
MH  - Mice
MH  - Polymorphism, Genetic
MH  - *Repetitive Sequences, Nucleic Acid
RF  - 21
EDAT- 1999/02/13 00:00
MHDA- 1999/02/13 00:01
CRDT- 1999/02/13 00:00
PHST- 1999/02/13 00:00 [pubmed]
PHST- 1999/02/13 00:01 [medline]
PHST- 1999/02/13 00:00 [entrez]
AID - 10.1093/jhered/90.1.39 [doi]
PST - ppublish
SO  - J Hered. 1999 Jan-Feb;90(1):39-42. doi: 10.1093/jhered/90.1.39.