PMID- 9987903
OWN - NLM
STAT- MEDLINE
DCOM- 19990318
LR  - 20190513
IS  - 0022-1503 (Print)
IS  - 0022-1503 (Linking)
VI  - 90
IP  - 1
DP  - 1999 Jan-Feb
TI  - Use of cosmid-derived and chromosome-specific canine microsatellites.
PG  - 52-4
AB  - The majority of microsatellite markers being used to generate the emerging 
      genetic linkage maps of the dog are derived from small-insert, random clones. 
      While such markers are easy to generate, they have the disadvantage that they 
      cannot easily be physically mapped by fluorescence in situ hybridization (FISH), 
      making it difficult to assess the extent of genome coverage represented by such 
      maps. In contrast, microsatellite markers from large-insert libraries enable the 
      linkage groups within which they fall to be physically anchored to specific 
      chromosomes. One aim of our work is to identify at least one 
      microsatellite-containing cosmid clone for each canine chromosome, to ensure that 
      linkage groups exist for all chromosomes. This is particularly important for a 
      species with as complex a karyotype as the dog. Locating two cosmids on each 
      chromosome would allow the orientation of the linkage groups to be established. 
      Chromosomal locations of cosmid clones containing microsatellites have been 
      determined by FISH and confirmed using canine chromosome-specific paints. 
      Microsatellite sequences have been genotyped on the DogMap reference family. 
      Microsatellites derived from flow-sorted, chromosome-specific libraries represent 
      another source of useful markers. Initial studies have been carried out on the 
      canine X chromosome, on which markers were underrepresented in our initial 
      studies.
FAU - Dickens, H F
AU  - Dickens HF
AD  - Centre for Preventive Medicine, Animal Health Trust, Suffolk, England.
FAU - Holmes, N G
AU  - Holmes NG
FAU - Ryder, E
AU  - Ryder E
FAU - Breen, M
AU  - Breen M
FAU - Thomas, R
AU  - Thomas R
FAU - Suter, N
AU  - Suter N
FAU - Sampson, J
AU  - Sampson J
FAU - Langford, C F
AU  - Langford CF
FAU - Ross, M
AU  - Ross M
FAU - Carter, N P
AU  - Carter NP
FAU - Binns, M M
AU  - Binns MM
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Review
PL  - United States
TA  - J Hered
JT  - The Journal of heredity
JID - 0375373
SB  - IM
MH  - Animals
MH  - *Chromosomes
MH  - *Cosmids
MH  - Dogs/*genetics
MH  - Female
MH  - Genetic Linkage
MH  - In Situ Hybridization, Fluorescence/veterinary
MH  - Male
MH  - *Microsatellite Repeats
MH  - X Chromosome
RF  - 17
EDAT- 1999/02/13 00:00
MHDA- 1999/02/13 00:01
CRDT- 1999/02/13 00:00
PHST- 1999/02/13 00:00 [pubmed]
PHST- 1999/02/13 00:01 [medline]
PHST- 1999/02/13 00:00 [entrez]
AID - 10.1093/jhered/90.1.52 [doi]
PST - ppublish
SO  - J Hered. 1999 Jan-Feb;90(1):52-4. doi: 10.1093/jhered/90.1.52.