PMID- 9988345
OWN - NLM
STAT- MEDLINE
DCOM- 19990324
LR  - 20200225
IS  - 0018-2214 (Print)
IS  - 0018-2214 (Linking)
VI  - 30
IP  - 11
DP  - 1998 Nov
TI  - Immunohistochemical video-microdensitometry of myocardial collagen type I and 
      type III.
PG  - 783-91
AB  - Collagen is an essential part of the cardiac interstitium. Collagen subtypes, 
      their location, total amount and the architecture of the fibrillar network are of 
      functional importance. Architecture in terms of density of the fibrillar network 
      is assumed to be reflected by the intensity of immunohistochemical staining of 
      collagen. The aim of this study was to evaluate a video-based microdensitometric 
      method for quantifying density expressed as absorbance of collagen subtypes I and 
      III stained with an indirect immunoperoxidase method in myectomy specimens of 
      patients with hypertrophic obstructive cardiomyopathy. Various factors 
      influencing the immunohistochemical staining product and the technical properties 
      of the image analysis system were investigated. Linearity between collagen 
      concentration and the absorbance of the immunohistochemical staining product was 
      demonstrated for collagen I using a dot-blot technique. Immunohistochemical 
      collagen staining and density measurement were easily reproducible. The cardiac 
      disability of the patients was assessed according to the New York Heart 
      Association (NYHA) criteria. There was a significant increase in collagen type I 
      density with higher NYHA class, whereas no significant association was found for 
      total collagen area fraction. Thus, video-based microdensitometry gives further 
      insight into the structural remodelling of myocardial collagens and reveals their 
      significance in the process of heart failure in hypertrophic cardiomyopathy.
FAU - Boerrigter, G
AU  - Boerrigter G
AD  - Department of Cardiology, Pneumology and Angiology, Heinrich-Heine-University 
      Dusseldorf, Germany.
FAU - Mundhenke, M
AU  - Mundhenke M
FAU - Stark, P
AU  - Stark P
FAU - Schulte, H D
AU  - Schulte HD
FAU - Strauer, B E
AU  - Strauer BE
FAU - Schwartzkopff, B
AU  - Schwartzkopff B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Histochem J
JT  - The Histochemical journal
JID - 0163161
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Cardiomyopathy, Hypertrophic/metabolism/pathology
MH  - Collagen/*analysis/metabolism
MH  - Computers
MH  - Densitometry
MH  - Female
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Middle Aged
MH  - Myocardium/*chemistry/metabolism
MH  - Software
MH  - Video Recording
EDAT- 1999/02/13 00:00
MHDA- 1999/02/13 00:01
CRDT- 1999/02/13 00:00
PHST- 1999/02/13 00:00 [pubmed]
PHST- 1999/02/13 00:01 [medline]
PHST- 1999/02/13 00:00 [entrez]
AID - 10.1023/a:1003492407387 [doi]
PST - ppublish
SO  - Histochem J. 1998 Nov;30(11):783-91. doi: 10.1023/a:1003492407387.