PMID- 9989288
OWN - NLM
STAT- MEDLINE
DCOM- 19990304
LR  - 20220310
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1436
IP  - 3
DP  - 1999 Jan 4
TI  - Tumor necrosis factor-alpha regulates in vivo expression of the rat UCP family 
      differentially.
PG  - 585-92
AB  - A family of uncoupling proteins (UCPs), free fatty acid anion transporters, plays 
      a crucial role in energy homeostatic thermoregulation. Tumor necrosis 
      factor-alpha (TNF-alpha), a member of the cytokine family, is well known as an 
      endogenous pyrogen. To evaluate the interaction of TNF-alpha with UCPs in 
      thermogenesis, effects of TNF-alpha on rat UCP gene expression were examined in 
      intrascapular brown adipose tissue (BAT), epididymal white adipose tissue (WAT) 
      and soleus muscle (Muscle). Administration of TNF-alpha elevated rectal 
      temperature by 0.7 degree C as well as serum leptin which peaked at 6 h, compared 
      with saline controls. BAT UCP1 mRNA expression was increased by 1.2-fold at 6 h 
      after the TNF-alpha treatment and decreased by 0.8-fold at 16 h after the 
      treatment. In contrast to UCP1 expression in BAT, UCP2 mRNA expressions in BAT, 
      WAT, and Muscle was increased to reach maximum levels of 1.3-, 1.6- and 1.8-fold, 
      respectively, at 16 h after the treatment. UCP3 mRNA in Muscle, but not in BAT or 
      WAT, was exclusively up-regulated by 1.7-fold at 16 h after the treatment. These 
      results indicate that TNF-alpha up-regulates UCP gene expression differentially 
      and tissue dependently, and add novel insights into thermogenesis under 
      conditions of malignancy and inflammation.
FAU - Masaki, T
AU  - Masaki T
AD  - Department of Internal Medicine 1, School of Medicine, Oita Medical University, 
      Japan.
FAU - Yoshimatsu, H
AU  - Yoshimatsu H
FAU - Chiba, S
AU  - Chiba S
FAU - Hidaka, S
AU  - Hidaka S
FAU - Tajima, D
AU  - Tajima D
FAU - Kakuma, T
AU  - Kakuma T
FAU - Kurokawa, M
AU  - Kurokawa M
FAU - Sakata, T
AU  - Sakata T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Probes)
RN  - 0 (Ion Channels)
RN  - 0 (Leptin)
RN  - 0 (Membrane Proteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Ucp1 protein, rat)
RN  - 0 (Ucp2 protein, rat)
RN  - 0 (Uncoupling Agents)
RN  - 0 (Uncoupling Protein 1)
RN  - 0 (Uncoupling Protein 2)
SB  - IM
MH  - Adipose Tissue/drug effects/metabolism
MH  - Adipose Tissue, Brown/drug effects/metabolism
MH  - Animals
MH  - Base Sequence
MH  - Body Temperature Regulation/drug effects
MH  - Carrier Proteins/*genetics
MH  - DNA Probes/genetics
MH  - Ion Channels
MH  - Leptin
MH  - Male
MH  - Membrane Proteins/*genetics
MH  - *Membrane Transport Proteins
MH  - *Mitochondrial Proteins
MH  - Muscle, Skeletal/drug effects/metabolism
MH  - Proteins/*genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Distribution
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Uncoupling Agents/*metabolism
MH  - Uncoupling Protein 1
MH  - Uncoupling Protein 2
MH  - Up-Regulation/drug effects
EDAT- 1999/02/16 00:00
MHDA- 1999/02/16 00:01
CRDT- 1999/02/16 00:00
PHST- 1999/02/16 00:00 [pubmed]
PHST- 1999/02/16 00:01 [medline]
PHST- 1999/02/16 00:00 [entrez]
AID - S0005-2760(98)00173-8 [pii]
AID - 10.1016/s0005-2760(98)00173-8 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Jan 4;1436(3):585-92. doi: 
      10.1016/s0005-2760(98)00173-8.