PMID- 9990127
OWN - NLM
STAT- MEDLINE
DCOM- 19990615
LR  - 20190513
IS  - 0021-924X (Print)
IS  - 0021-924X (Linking)
VI  - 125
IP  - 2
DP  - 1999 Feb
TI  - Comparison of the apoptotic pathways induced by L-amino acid oxidase and hydrogen 
      peroxide.
PG  - 305-9
AB  - We have found that L-amino acid oxidase (LAO) induces apoptosis in several 
      cultured cell lines by generating H2O2 [Suhr, S.M. and Kim, D.S. (1996) Biochem. 
      Biophys. Res. Commun. 224, 134-139]. It is demonstrated in the present work that 
      the LAO-induced apoptotic mechanism is clearly distinguished from the one 
      stimulated directly by exogenous H2O2. MOLT-4 cells undergo somewhat different 
      morphological changes depending on the apoptotic inducer, LAO or H2O2. 
      LAO-induced apoptosis can be protected by the antioxidant N-acetylcysteine or the 
      free radical scavenger melatonin, while H2O2-induced apoptotic cell death is not 
      protected. A caspase inhibitor, acetyl-Tyr-Val-Ala-Asp-aldehyde 
      (ac-YVAD-aldehyde), prevents cell death when the apoptosis is induced by 
      exogenous H2O2. On the other hand, the ac-YVAD-aldehyde tetrapeptide inhibitor 
      that is dominantly effective on interleukin-1beta converting enzyme failed to 
      block the apoptotic event initiated by LAO. Several lines of experimental 
      evidence suggest that apoptotic cell death induced by LAO is not due solely to 
      the hydrogen peroxide produced by the enzymatic reaction.
FAU - Suhr, S M
AU  - Suhr SM
AD  - Department of Biochemistry, College of Science, and Bioproducts Research Center, 
      Yonsei University, Seoul, 120-749, Korea.
FAU - Kim, D S
AU  - Kim DS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Biochem
JT  - Journal of biochemistry
JID - 0376600
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.4.- (Amino Acid Oxidoreductases)
RN  - EC 1.4.3.2 (L-Amino Acid Oxidase)
SB  - IM
MH  - Amino Acid Oxidoreductases/*pharmacology
MH  - *Apoptosis
MH  - Cell Cycle/drug effects
MH  - Flow Cytometry
MH  - Humans
MH  - Hydrogen Peroxide/*pharmacology
MH  - L-Amino Acid Oxidase
MH  - Tumor Cells, Cultured
EDAT- 1999/02/17 00:00
MHDA- 1999/02/17 00:01
CRDT- 1999/02/17 00:00
PHST- 1999/02/17 00:00 [pubmed]
PHST- 1999/02/17 00:01 [medline]
PHST- 1999/02/17 00:00 [entrez]
AID - 10.1093/oxfordjournals.jbchem.a022287 [doi]
PST - ppublish
SO  - J Biochem. 1999 Feb;125(2):305-9. doi: 10.1093/oxfordjournals.jbchem.a022287.