PMID- 9990483
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20191102
IS  - 1052-9551 (Print)
IS  - 1052-9551 (Linking)
VI  - 7
IP  - 5
DP  - 1998 Oct
TI  - Cyclin D1 gene amplification and p16 gene deletion in patients with esophageal 
      carcinosarcoma.
PG  - 253-9
AB  - Cyclin D1 (CD1) gene amplification is frequently observed in esophageal 
      carcinosarcoma by differential polymerase chain reaction (DPCR). In this study, 
      fluorescence in situ hybridization (FISH) was performed to show more direct 
      evidence of CD1 gene amplification in patients with esophageal carcinosarcoma. 
      FISH results were also compared with DPCR results studied previously. FISH 
      analysis revealed CD1 gene amplification in all four patients with esophageal 
      carcinosarcoma. CD1 gene amplification occurred with a high incidence in both 
      components of esophageal carcinosarcoma, suggesting that CD1 gene amplification 
      could have an important role in malignant transformation processes of esophageal 
      carcinosarcoma. The results of the current study also suggest that FISH is a more 
      sensitive method than DPCR. Because inactivation of p16 gene (which is a putative 
      tumor suppressor gene) is thought to have similar oncogenic effects with CD1 gene 
      amplification, DPCR was used to examine whether p16 homozygous deletion occurs in 
      esophageal carcinosarcoma. These results suggest that homozygous deletion of the 
      p16 gene occurs less frequently than CD1 gene amplification in esophageal 
      carcinosarcoma. It does not seem to be an alternative event to CD1 gene 
      amplification, though the number of studied cases was small.
FAU - Suzuki, H
AU  - Suzuki H
AD  - Second Department of Pathology, Hokkaido University School of Medicine, Sapporo, 
      Japan.
FAU - Fujioka, Y
AU  - Fujioka Y
FAU - Nagashima, K
AU  - Nagashima K
LA  - eng
SI  - GENBANK/K00046
SI  - GENBANK/S69804
SI  - GENBANK/Y00817
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
RN  - 0 (DNA Primers)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinosarcoma/*genetics/pathology
MH  - Cyclin D1/*genetics
MH  - DNA Primers/chemistry
MH  - Esophageal Neoplasms/*genetics/pathology
MH  - Gene Amplification
MH  - Gene Deletion
MH  - Genes, p16/*genetics
MH  - Homozygote
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
EDAT- 1999/02/17 00:00
MHDA- 1999/02/17 00:01
CRDT- 1999/02/17 00:00
PHST- 1999/02/17 00:00 [pubmed]
PHST- 1999/02/17 00:01 [medline]
PHST- 1999/02/17 00:00 [entrez]
AID - 10.1097/00019606-199810000-00004 [doi]
PST - ppublish
SO  - Diagn Mol Pathol. 1998 Oct;7(5):253-9. doi: 10.1097/00019606-199810000-00004.