PMID- 10228168
OWN - NLM
STAT- MEDLINE
DCOM- 19990628
LR  - 20211203
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 9
DP  - 1999 May 4
TI  - A WW domain-containing yes-associated protein (YAP) is a novel transcriptional 
      co-activator.
PG  - 2551-62
AB  - A protein module called the WW domain recognizes and binds to a short 
      oligopeptide called the PY motif, PPxY, to mediate protein-protein interactions. 
      The PY motif is present in the transcription activation domains of a wide range 
      of transcription factors including c-Jun, AP-2, NF-E2, C/EBPalpha and PEBP2/CBF, 
      suggesting that it plays an important role in transcriptional activation. We show 
      here that mutation of the PY motif in the subregion of the activation domain of 
      the DNA-binding subunit of PEBP2, PEBP2alpha, abolishes its transactivation 
      function. Using yeast two-hybrid screening, we demonstrate that Yes-associated 
      protein (YAP) binds to the PY motif of PEBP2alpha through its WW domain. The 
      C-terminal region of YAP fused to the DNA-binding domain of GAL4 showed 
      transactivation as strong as that of GAL4-VP16. Exogenously expressed YAP 
      conferred transcription-stimulating activity on the PY motif fused to the GAL4 
      DNA-binding domain as well as to native PEBP2alpha. The osteocalcin promoter was 
      stimulated by exogenous PEBP2alphaA and a dominant negative form of YAP strongly 
      inhibited this activity, suggesting YAP involvement in this promoter activity in 
      vivo. These results indicate that the PY motif is a novel transcription 
      activation domain that functions by recruiting YAP as a strong transcription 
      activator to target genes.
FAU - Yagi, R
AU  - Yagi R
AD  - Department of Viral Oncology, Institute for Virus Research, Kyoto University, 
      Shogo-in, Sakyo-ku, Kyoto 606-8507, Japan.
FAU - Chen, L F
AU  - Chen LF
FAU - Shigesada, K
AU  - Shigesada K
FAU - Murakami, Y
AU  - Murakami Y
FAU - Ito, Y
AU  - Ito Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (GAL4 protein, S cerevisiae)
RN  - 0 (Phosphoproteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factor AP-2)
RN  - 0 (Transcription Factors)
RN  - 0 (YAP-Signaling Proteins)
RN  - 0 (YAP1 protein, human)
RN  - 0 (Yap1 protein, mouse)
RN  - 104982-03-8 (Osteocalcin)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carrier Proteins/isolation & purification/*metabolism
MH  - Cell Compartmentation
MH  - Cell Cycle Proteins
MH  - Cell Nucleus
MH  - Cytoplasm
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Fungal Proteins/genetics/*metabolism
MH  - Humans
MH  - Mice
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Osteocalcin/genetics
MH  - Phosphoproteins/isolation & purification/*metabolism
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Recombinant Fusion Proteins/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Transcription Factor AP-2
MH  - Transcription Factors/genetics/*metabolism
MH  - *Transcription, Genetic
MH  - *Transcriptional Activation
MH  - YAP-Signaling Proteins
PMC - PMC1171336
EDAT- 1999/05/06 00:00
MHDA- 1999/05/06 00:01
PMCR- 2000/05/04
CRDT- 1999/05/06 00:00
PHST- 1999/05/06 00:00 [pubmed]
PHST- 1999/05/06 00:01 [medline]
PHST- 1999/05/06 00:00 [entrez]
PHST- 2000/05/04 00:00 [pmc-release]
AID - 10.1093/emboj/18.9.2551 [doi]
PST - ppublish
SO  - EMBO J. 1999 May 4;18(9):2551-62. doi: 10.1093/emboj/18.9.2551.