PMID- 10360813
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20220223
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 82
IP  - 1
DP  - 1999 Jul 2
TI  - Microglial/macrophage expression of interleukin 10 in human glioblastomas.
PG  - 12-6
AB  - Interleukin 10 (IL-10) expression has been found to be correlated with the extent 
      of malignancy in gliomas. In vitro, IL-10 increases proliferation and migratory 
      capacity in human glioma cell lines. In this study, we localized the site of 
      IL-10 synthesis in gliomas to cells of microglial origin. Biopsy specimens from 
      11 patients with malignant glioma were processed on native tissues and at early 
      cell culture passages (0-4). IL-10 mRNA was analyzed by RT-PCR and in situ 
      hybridization. Protein was quantitatively assessed by ELISA in cell culture 
      supernatants, and cells expressing IL-10 were determined by a combination of 
      immunohistochemistry for CD68 (specific for microglia/macrophage lineage) and 
      IL-10 in situ hybridization. IL-10 mRNA decreased from passage 0 to 4 in all 
      samples and was undetectable beyond passage 5. Such downregulation of mRNA leads 
      to a steep decrease of IL-10 protein in culture supernatants (below detection 
      level, 0.05 ng/ml, beyond passage 1). The combination of in situ hybridization 
      for IL-10 and CD68 immunostaining revealed that only cells of the 
      microglia/macrophage lineage produced IL-10 mRNA. Our results identify 
      microglia/macrophage cells as the major source of IL-10 expression in gliomas 
      which decreases markedly during early passages of primary cultures of human 
      gliomas due to a progressive reduction of microglia/macrophages present.
FAU - Wagner, S
AU  - Wagner S
AD  - Department of Neurosurgery, University of Wurzburg, Germany.
FAU - Czub, S
AU  - Czub S
FAU - Greif, M
AU  - Greif M
FAU - Vince, G H
AU  - Vince GH
FAU - Suss, N
AU  - Suss N
FAU - Kerkau, S
AU  - Kerkau S
FAU - Rieckmann, P
AU  - Rieckmann P
FAU - Roggendorf, W
AU  - Roggendorf W
FAU - Roosen, K
AU  - Roosen K
FAU - Tonn, J C
AU  - Tonn JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD68 antigen, human)
RN  - 0 (RNA, Messenger)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, CD/analysis
MH  - Antigens, Differentiation, Myelomonocytic/analysis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Glioblastoma/*metabolism
MH  - Humans
MH  - Interleukin-10/*biosynthesis/genetics
MH  - Macrophages/*metabolism
MH  - Male
MH  - Microglia/*metabolism
MH  - Middle Aged
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 1999/06/09 10:00
MHDA- 2000/06/20 09:00
CRDT- 1999/06/09 10:00
PHST- 1999/06/09 10:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/06/09 10:00 [entrez]
AID - 10.1002/(SICI)1097-0215(19990702)82:1<12::AID-IJC3>3.0.CO;2-O [pii]
AID - 10.1002/(sici)1097-0215(19990702)82:1<12::aid-ijc3>3.0.co;2-o [doi]
PST - ppublish
SO  - Int J Cancer. 1999 Jul 2;82(1):12-6. doi: 
      10.1002/(sici)1097-0215(19990702)82:1<12::aid-ijc3>3.0.co;2-o.