PMID- 10380923
OWN - NLM
STAT- MEDLINE
DCOM- 19990722
LR  - 20220825
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 97
IP  - 6
DP  - 1999 Jun 11
TI  - Subventricular zone astrocytes are neural stem cells in the adult mammalian 
      brain.
PG  - 703-16
AB  - Neural stem cells reside in the subventricular zone (SVZ) of the adult mammalian 
      brain. This germinal region, which continually generates new neurons destined for 
      the olfactory bulb, is composed of four cell types: migrating neuroblasts, 
      immature precursors, astrocytes, and ependymal cells. Here we show that SVZ 
      astrocytes, and not ependymal cells, remain labeled with proliferation markers 
      after long survivals in adult mice. After elimination of immature precursors and 
      neuroblasts by an antimitotic treatment, SVZ astrocytes divide to generate 
      immature precursors and neuroblasts. Furthermore, in untreated mice, SVZ 
      astrocytes specifically infected with a retrovirus give rise to new neurons in 
      the olfactory bulb. Finally, we show that SVZ astrocytes give rise to cells that 
      grow into multipotent neurospheres in vitro. We conclude that SVZ astrocytes act 
      as neural stem cells in both the normal and regenerating brain.
FAU - Doetsch, F
AU  - Doetsch F
AD  - The Rockefeller University, New York, New York 10021, USA.
FAU - Caille, I
AU  - Caille I
FAU - Lim, D A
AU  - Lim DA
FAU - Garcia-Verdugo, J M
AU  - Garcia-Verdugo JM
FAU - Alvarez-Buylla, A
AU  - Alvarez-Buylla A
LA  - eng
GR  - GM07739/GM/NIGMS NIH HHS/United States
GR  - HD32116/HD/NICHD NIH HHS/United States
GR  - NS28478/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Glial Fibrillary Acidic Protein)
SB  - IM
MH  - Animals
MH  - Astrocytes/*cytology
MH  - Brain/cytology/physiology
MH  - Cerebral Ventricles/*cytology/physiology
MH  - Chick Embryo
MH  - Glial Fibrillary Acidic Protein/genetics/metabolism
MH  - Male
MH  - Mice
MH  - Olfactory Bulb
MH  - Regeneration
MH  - Stem Cells/*cytology
EDAT- 1999/06/25 00:00
MHDA- 1999/06/25 00:01
CRDT- 1999/06/25 00:00
PHST- 1999/06/25 00:00 [pubmed]
PHST- 1999/06/25 00:01 [medline]
PHST- 1999/06/25 00:00 [entrez]
AID - S0092-8674(00)80783-7 [pii]
AID - 10.1016/s0092-8674(00)80783-7 [doi]
PST - ppublish
SO  - Cell. 1999 Jun 11;97(6):703-16. doi: 10.1016/s0092-8674(00)80783-7.