PMID- 10455434
OWN - NLM
STAT- MEDLINE
DCOM- 20000224
LR  - 20240104
IS  - 0969-7128 (Print)
IS  - 0969-7128 (Linking)
VI  - 6
IP  - 7
DP  - 1999 Jul
TI  - Hydrodynamics-based transfection in animals by systemic administration of plasmid 
      DNA.
PG  - 1258-66
AB  - Development of methods that allow an efficient expression of exogenous genes in 
      animals would provide tools for gene function studies, treatment of diseases and 
      for obtaining gene products. Therefore, we have developed a hydrodynamics-based 
      procedure for expressing transgenes in mice by systemic administration of plasmid 
      DNA. Using cDNA of luciferase and beta-galactosidase as a reporter gene, we 
      demonstrated that an efficient gene transfer and expression can be achieved by a 
      rapid injection of a large volume of DNA solution into animals via the tail vein. 
      Among the organs expressing the transgene, the liver showed the highest level of 
      gene expression. As high as 45 microg of luciferase protein per gram of liver can 
      be achi- eved by a single tail vein injection of 5 microg of plasmid DNA into a 
      mouse. Histochemical analysis using beta-galactosidase gene as a reporter reveals 
      that approximately 40percent of hepatocytes express the transgene. The 
      time-response curve shows that the level of transgene expression in the liver 
      reaches the peak level in approximately 8 h after injection and decreases 
      thereafter. The peak level of gene expression can be regained by repeated 
      injection of plasmid DNA. These results suggest that a simple, convenient and 
      efficient method has been developed and which can be used as an effective means 
      for studying gene function, gene regulation and molecular pathophysiology through 
      gene transfer, as well as for expressing proteins in animals.
FAU - Liu, F
AU  - Liu F
AD  - Department of Pharmaceutical Sciences, School of Pharmacy, University of 
      Pittsburgh, Pittsburgh, PA 15261, USA.
FAU - Song, Y
AU  - Song Y
FAU - Liu, D
AU  - Liu D
LA  - eng
GR  - CA72529/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Gene Ther
JT  - Gene therapy
JID - 9421525
RN  - 9007-49-2 (DNA)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - DNA/*administration & dosage/metabolism
MH  - Gene Expression
MH  - Genetic Therapy/*methods
MH  - Injections, Intravenous
MH  - Liver/metabolism
MH  - Luciferases/analysis/*genetics
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Time Factors
MH  - Transfection/*methods
EDAT- 1999/08/24 10:00
MHDA- 2000/02/26 09:00
CRDT- 1999/08/24 10:00
PHST- 1999/08/24 10:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 1999/08/24 10:00 [entrez]
AID - 10.1038/sj.gt.3300947 [doi]
PST - ppublish
SO  - Gene Ther. 1999 Jul;6(7):1258-66. doi: 10.1038/sj.gt.3300947.