PMID- 10749121
OWN - NLM
STAT- MEDLINE
DCOM- 20000504
LR  - 20081121
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 60
IP  - 6
DP  - 2000 Mar 15
TI  - Coordinate up-regulation of Sp1 DNA-binding activity and urokinase receptor 
      expression in breast carcinoma.
PG  - 1546-51
AB  - The regulatory mechanisms underlying the overexpression of urokinase-type 
      plasminogen activator (uPA) and its receptor (uPAR) in highly invasive breast 
      carcinomas remain poorly understood. In this study, we have simultaneously 
      determined the level of uPAR and the activity of the transcription factor Sp1 in 
      14 breast carcinomas and 5 benign lesions. We found that uPAR levels and 
      Sp1-binding activity are coordinately elevated in malignant tumors (r, 0.94; P < 
      0.001). On the contrary, undetectable or only barely detectable levels of uPAR 
      and Sp1 activity were found in benign breast lesions. Finally, the engagement of 
      uPAR by catalytically inactive uPA in the MDA-MB-231 breast carcinoma cell line 
      results in a rapid up-regulation of Sp1-binding activity followed by an increase 
      of uPAR protein. These results, taken together, suggest the existence of a 
      uPA-dependent positive regulatory loop that may progressively enhance malignant 
      breast cell invasiveness.
FAU - Zannetti, A
AU  - Zannetti A
AD  - Centro Medicina Nucleare, Consiglio Nazionale delle Ricerche, Dipartimento di 
      Scienze Biomorfologiche e Funzionali, Universita Federico II, Naples, Italy.
FAU - Del Vecchio, S
AU  - Del Vecchio S
FAU - Carriero, M V
AU  - Carriero MV
FAU - Fonti, R
AU  - Fonti R
FAU - Franco, P
AU  - Franco P
FAU - Botti, G
AU  - Botti G
FAU - D'Aiuto, G
AU  - D'Aiuto G
FAU - Stoppelli, M P
AU  - Stoppelli MP
FAU - Salvatore, M
AU  - Salvatore M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Oligonucleotides)
RN  - 0 (PLAUR protein, human)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Urokinase Plasminogen Activator)
RN  - 0 (Sp1 Transcription Factor)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Blotting, Western
MH  - Breast/chemistry/pathology
MH  - Breast Neoplasms/*metabolism/pathology
MH  - DNA-Binding Proteins/metabolism
MH  - Female
MH  - HeLa Cells
MH  - Humans
MH  - Immunohistochemistry
MH  - Middle Aged
MH  - Oligonucleotides/metabolism
MH  - Protein Binding
MH  - Receptors, Cell Surface/*biosynthesis
MH  - Receptors, Urokinase Plasminogen Activator
MH  - Sp1 Transcription Factor/*metabolism
MH  - Tumor Cells, Cultured/drug effects/metabolism
MH  - Up-Regulation/drug effects
MH  - Urokinase-Type Plasminogen Activator/pharmacology
EDAT- 2000/04/05 09:00
MHDA- 2000/05/08 09:00
CRDT- 2000/04/05 09:00
PHST- 2000/04/05 09:00 [pubmed]
PHST- 2000/05/08 09:00 [medline]
PHST- 2000/04/05 09:00 [entrez]
PST - ppublish
SO  - Cancer Res. 2000 Mar 15;60(6):1546-51.