PMID- 10880451
OWN - NLM
STAT- MEDLINE
DCOM- 20001005
LR  - 20231105
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 19
IP  - 13
DP  - 2000 Jul 3
TI  - Two RING finger proteins mediate cooperation between ubiquitin-conjugating 
      enzymes in DNA repair.
PG  - 3388-97
AB  - Two ubiquitin-conjugating enzymes, RAD6 and the heteromeric UBC13-MMS2 complex, 
      have been implicated in post-replicative DNA damage repair in yeast. Here we 
      provide a mechanistic basis for cooperation between the two enzymes. We show that 
      two chromatin-associated RING finger proteins, RAD18 and RAD5, play a central 
      role in mediating physical contacts between the members of the RAD6 pathway. RAD5 
      recruits the UBC13-MMS2 complex to DNA by means of its RING finger domain. 
      Moreover, RAD5 association with RAD18 brings UBC13-MMS2 into contact with the 
      RAD6-RAD18 complex. Interaction between the two RING finger proteins thus 
      promotes the formation of a heteromeric complex in which the two distinct 
      ubiquitin-conjugating activities of RAD6 and UBC13-MMS2 can be closely 
      coordinated. Surprisingly, UBC13 and MMS2 are largely cytosolic proteins, but DNA 
      damage triggers their redistribution to the nucleus. These findings suggest a 
      mechanism by which the activity of this DNA repair pathway could be regulated.
FAU - Ulrich, H D
AU  - Ulrich HD
AD  - Department of Molecular Cell Biology, Max Planck Institute for Biochemistry, Am 
      Klopferspitz 18a, 82152 Martinsried, Germany.
FAU - Jentsch, S
AU  - Jentsch S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Biopolymers)
RN  - 0 (Chromatin)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (MMS2 protein, S cerevisiae)
RN  - 0 (RAD18 protein, S cerevisiae)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 2.3.2.23 (RAD6 protein, S cerevisiae)
RN  - EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.1.- (RAD5 protein, S cerevisiae)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 6.- (Ligases)
SB  - IM
MH  - *Adenosine Triphosphatases
MH  - Biopolymers
MH  - Cell Nucleus/metabolism
MH  - Chromatin/metabolism
MH  - Cytoplasm/metabolism
MH  - DNA Damage
MH  - DNA Helicases
MH  - *DNA Repair
MH  - DNA-Binding Proteins/*metabolism
MH  - Fungal Proteins/*metabolism
MH  - Ligases/*metabolism
MH  - Protein Binding
MH  - Saccharomyces cerevisiae/enzymology/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Ubiquitin-Conjugating Enzymes
MH  - Ubiquitin-Protein Ligases
PMC - PMC313941
EDAT- 2000/07/06 11:00
MHDA- 2000/10/07 11:01
PMCR- 2001/07/03
CRDT- 2000/07/06 11:00
PHST- 2000/07/06 11:00 [pubmed]
PHST- 2000/10/07 11:01 [medline]
PHST- 2000/07/06 11:00 [entrez]
PHST- 2001/07/03 00:00 [pmc-release]
AID - cdd324 [pii]
AID - 10.1093/emboj/19.13.3388 [doi]
PST - ppublish
SO  - EMBO J. 2000 Jul 3;19(13):3388-97. doi: 10.1093/emboj/19.13.3388.