PMID- 10925317
OWN - NLM
STAT- MEDLINE
DCOM- 20000914
LR  - 20190515
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 165
IP  - 4
DP  - 2000 Aug 15
TI  - CD4 and CD8 T cells have redundant but not identical roles in virus-induced 
      demyelination.
PG  - 2278-86
AB  - A chronic demyelinating disease results from murine infection with the 
      neurotropic strain JHM of mouse hepatitis virus (MHV-JHM). Demyelination is 
      largely immune mediated. In this study, the individual roles of CD4 and CD8 T 
      cells in MHV-induced demyelination were investigated using 
      recombination-activating gene 1-/- (RAG1-/-) mice infected with an attenuated 
      strain of MHV-JHM. These animals develop demyelination only after adoptive 
      transfer of splenocytes from mice previously immunized to MHV. In this study, we 
      show that, following adoptive transfer, virus-specific CD4 and CD8 T cells 
      rapidly infiltrate the CNS of MHV-JHM-infected RAG1-/- mice. Adoptive transfer of 
      CD4 T cell-enriched donors resulted in more severe clinical disease accompanied 
      by less demyelination than was detected in the recipients of undepleted cells. 
      Macrophage infiltration into the gray matter of CD4 T cell-enriched recipients 
      was greater than that observed in mice receiving undepleted splenocytes. In 
      contrast, CD8 T cell-enriched recipients developed delayed disease with extensive 
      demyelination of the spinal cord. MHV-JHM-infected RAG1-/- mice receiving donors 
      depleted of both CD4 and CD8 T cells did not develop demyelination. These results 
      demonstrate that the development of demyelination following MHV infection may be 
      initiated by either CD4 or CD8 T cells. Furthermore, they show that CD4 T cells 
      contribute more prominently than CD8 T cells to the severity of clinical disease, 
      and that this correlates with increased macrophage infiltration into the gray 
      matter.
FAU - Wu, G F
AU  - Wu GF
AD  - Program in Neuroscience, Departments of Pediatrics and Microbiology, and 
      University of Iowa College of Medicine, University of Iowa, Iowa City, IA 52242, 
      USA.
FAU - Dandekar, A A
AU  - Dandekar AA
FAU - Pewe, L
AU  - Pewe L
FAU - Perlman, S
AU  - Perlman S
LA  - eng
GR  - MH2066-03/MH/NIMH NIH HHS/United States
GR  - NS36592/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Epitopes, T-Lymphocyte)
SB  - IM
MH  - Adoptive Transfer/methods
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/*immunology/pathology/transplantation
MH  - CD8-Positive T-Lymphocytes/*immunology/pathology/transplantation
MH  - Cell Movement/immunology
MH  - Coronavirus Infections/*immunology/pathology/*virology
MH  - Demyelinating Diseases/*immunology/pathology/*virology
MH  - Disease Models, Animal
MH  - Epitopes, T-Lymphocyte/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Murine hepatitis virus/*immunology
MH  - Spinal Cord/immunology/pathology
MH  - T-Lymphocyte Subsets/immunology/pathology/transplantation
MH  - Viral Load
EDAT- 2000/08/05 11:00
MHDA- 2000/09/19 11:01
CRDT- 2000/08/05 11:00
PHST- 2000/08/05 11:00 [pubmed]
PHST- 2000/09/19 11:01 [medline]
PHST- 2000/08/05 11:00 [entrez]
AID - ji_v165n4p2278 [pii]
AID - 10.4049/jimmunol.165.4.2278 [doi]
PST - ppublish
SO  - J Immunol. 2000 Aug 15;165(4):2278-86. doi: 10.4049/jimmunol.165.4.2278.