PMID- 10958786
OWN - NLM
STAT- MEDLINE
DCOM- 20000907
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 289
IP  - 5483
DP  - 2000 Aug 25
TI  - Function of an axonal chemoattractant modulated by metalloprotease activity.
PG  - 1365-7
AB  - The axonal chemoattractant netrin-1 guides spinal commissural axons by activating 
      its receptor DCC (Deleted in Colorectal Cancer). We have found that chemical 
      inhibitors of metalloproteases potentiate netrin-mediated axon outgrowth in 
      vitro. We have also found that DCC is a substrate for metalloprotease-dependent 
      ectodomain shedding, and that the inhibitors block proteolytic processing of DCC 
      and cause an increase in DCC protein levels on axons within spinal cord explants. 
      Thus, potentiation of netrin activity by inhibitors may result from stabilization 
      of DCC on the axons, and proteolytic activity may regulate axon migration by 
      controlling the number of functional extracellular axon guidance receptors.
FAU - Galko, M J
AU  - Galko MJ
AD  - Department of Anatomy, Howard Hughes Medical Institute, University of California, 
      San Francisco, CA 94143, USA.
FAU - Tessier-Lavigne, M
AU  - Tessier-Lavigne M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Ntn1 protein, rat)
RN  - 0 (Phenanthrolines)
RN  - 0 (Protease Inhibitors)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 158651-98-0 (Netrin-1)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - W4X6ZO7939 (1,10-phenanthroline)
SB  - IM
CIN - Science. 2000 Aug 25;289(5483):1308-10. PMID: 10979856
MH  - Animals
MH  - Axons/*physiology
MH  - CHO Cells
MH  - Cell Adhesion Molecules/chemistry/*metabolism
MH  - Cricetinae
MH  - Culture Techniques
MH  - Growth Cones/physiology
MH  - Metalloendopeptidases/antagonists & inhibitors/*metabolism
MH  - Nerve Growth Factors/*metabolism
MH  - Netrin-1
MH  - Phenanthrolines/pharmacology
MH  - Protease Inhibitors/pharmacology
MH  - Rats
MH  - Spinal Cord/*cytology/*enzymology/metabolism
MH  - *Tumor Suppressor Proteins
EDAT- 2000/08/26 11:00
MHDA- 2000/09/09 11:01
CRDT- 2000/08/26 11:00
PHST- 2000/08/26 11:00 [pubmed]
PHST- 2000/09/09 11:01 [medline]
PHST- 2000/08/26 11:00 [entrez]
AID - 8774 [pii]
AID - 10.1126/science.289.5483.1365 [doi]
PST - ppublish
SO  - Science. 2000 Aug 25;289(5483):1365-7. doi: 10.1126/science.289.5483.1365.