PMID- 10975656
OWN - NLM
STAT- MEDLINE
DCOM- 20010111
LR  - 20240109
IS  - 0894-0282 (Print)
IS  - 0894-0282 (Linking)
VI  - 13
IP  - 9
DP  - 2000 Sep
TI  - Proteome analysis of differentially displayed proteins as a tool for the 
      investigation of symbiosis.
PG  - 995-1009
AB  - Two-dimensional gel electrophoresis was used to identify differentially displayed 
      proteins expressed during the symbiotic interaction between the bacterium 
      Sinorhizobium meliloti strain 1021 and the legume Melilotus alba (white 
      sweetclover). Our aim was to characterize novel symbiosis proteins and to 
      determine how the two symbiotic partners alter their respective metabolisms as 
      part of the interaction, by identifying gene products that are differentially 
      present between the symbiotic and non-symbiotic states. Proteome maps from 
      control M. alba roots, wild-type nodules, cultured S. meliloti, and S. meliloti 
      bacteroids were generated and compared. Over 250 proteins were induced or 
      up-regulated in the nodule, compared with the root, and over 350 proteins were 
      down-regulated in the bacteroid form of the rhizobia, compared with cultured 
      cells. N-terminal amino acid sequencing and matrix-assisted laser 
      desorption/ionization time-of-flight mass spectrometry peptide mass fingerprint 
      analysis, in conjunction with data base searching, were used to assign putative 
      identity to nearly 100 nodule, bacterial, and bacteroid proteins. These included 
      the previously identified nodule proteins leghemoglobin and NifH as well as 
      proteins involved in carbon and nitrogen metabolism in S. meliloti. Bacteroid 
      cells showed down-regulation of several proteins involved in nitrogen 
      acquisition, including glutamine synthetase, urease, a urea-amide binding 
      protein, and a PII isoform, indicating that the bacteroids were nitrogen 
      proficient. The down-regulation of several enzymes involved in 
      polyhydroxybutyrate synthesis and a cell division protein was also observed. This 
      work shows that proteome analysis will be a useful strategy to link sequence 
      information and functional genomics.
FAU - Natera, S H
AU  - Natera SH
AD  - Plant-Microbe Interaction Group, Research School of Biological Sciences, 
      Australian National University, Canberra City.
FAU - Guerreiro, N
AU  - Guerreiro N
FAU - Djordjevic, M A
AU  - Djordjevic MA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Plant Microbe Interact
JT  - Molecular plant-microbe interactions : MPMI
JID - 9107902
RN  - 0 (Proteome)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Fabaceae/*genetics/metabolism/microbiology
MH  - Nitrogen/metabolism
MH  - *Plants, Medicinal
MH  - *Proteome
MH  - Sinorhizobium meliloti/*genetics/metabolism
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - *Symbiosis
EDAT- 2000/09/07 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/07 11:00
PHST- 2000/09/07 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/07 11:00 [entrez]
AID - 10.1094/MPMI.2000.13.9.995 [doi]
PST - ppublish
SO  - Mol Plant Microbe Interact. 2000 Sep;13(9):995-1009. doi: 
      10.1094/MPMI.2000.13.9.995.