PMID- 11185708
OWN - NLM
STAT- MEDLINE
DCOM- 20010301
LR  - 20181113
IS  - 0169-4197 (Print)
IS  - 0169-4197 (Linking)
VI  - 28
IP  - 1
DP  - 2000 Aug
TI  - Angiogenesis and cathepsin expression are prognostic factors in pancreatic 
      adenocarcinoma after curative resection.
PG  - 31-9
AB  - BACKGROUND: Curative resection of pancreatic adenocarcinoma is the only clinical 
      parameter related to a favorable prognosis while other clinicopathological 
      parameters fail. To evaluate whether angiogenesis, vascular endothelial growth 
      factor (VEGF) or certain tumor proteases, e.g., cathepsin B (CTSB) and L (CTSL), 
      are factors of prognostic relevance, we investigated their expression in patients 
      with long- and short-term survival after curative resection (RO) because of 
      pancreatic adenocarcinoma. METHODS: Twenty-nine tissue samples from patients with 
      adenocarcinoma of the pancreas were examined. The patients were selected in a 
      long-term survival group with a survival > or = 24 mo (n = 18) and a shortterm 
      survival group of patients, who died within 8 mo after surgery because of their 
      malignancy (n = 11). The microvessel quantification was performed 
      immunohistochemically using a monoclonal anti-CD34 antibody. VEGF, CTSB, and CTSL 
      expressions was studied using polyclonal antibodies (PAbs). RESULTS: The median 
      microvessel density (MVD) was 75 (range 39-182). MVD correlated significantly 
      with the survival time after surgery (p = 0.0132) but not with 
      clinicopathological parameters. In cancer cells, VEGF was positive in 82.8% and 
      showed significant correlation with the MVD (p = 0.0002) and survival time (p = 
      0.0395). Positive immunoreactivity could be obtained for 96.5% for CTSB and 84.2% 
      for CTSL. Expression of both proteases correlated significantly with the survival 
      time after surgery (CTSB p = 0.0002, CTSL p = 0.0001). Furthermore, CTSB 
      expression correlated with invasion of the perineural space. Thus, a short 
      postoperative survival correlated with a high MVD, and highly expressed VEGF, 
      CTSB, and CTSL. No significant correlation between MVD, VEGF, as well as CTSL and 
      clinicopathological parameters was found. For routinely assessed markers (e.g., 
      TNM-stage, UICC-stage, and so on) no significant correlation with survival time 
      was found in this small group of patients. CONCLUSION: These findings indicate 
      that the MVD, VEGF, CTSB, and CTSL are prognostic factors after curative 
      resection, whereas other parameters (TNM, UICC, and so on) failed to show 
      prognostic relevance in our group of patients. Furthermore, the correlation 
      between MVD and VEGF underlines the importance of this growth factor for 
      angiogenesis and tumor growth. The correlation between CTSB and perineural 
      invasion demonstrates the involvement of cathepsins in local tumor invasion.
FAU - Niedergethmann, M
AU  - Niedergethmann M
AD  - Department of Surgery, University-Hospital Mannheim, University of Heidelberg, 
      Germany. marco.niedergethmann@chir.ma.uni-heidelberg.de
FAU - Hildenbrand, R
AU  - Hildenbrand R
FAU - Wolf, G
AU  - Wolf G
FAU - Verbeke, C S
AU  - Verbeke CS
FAU - Richter, A
AU  - Richter A
FAU - Post, S
AU  - Post S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Int J Pancreatol
JT  - International journal of pancreatology : official journal of the International 
      Association of Pancreatology
JID - 8703511
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Lymphokines)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
RN  - EC 3.4.- (Cathepsins)
SB  - IM
MH  - Adenocarcinoma/*blood supply/*metabolism/mortality/surgery
MH  - Aged
MH  - Biomarkers, Tumor/*metabolism
MH  - Cathepsins/*metabolism
MH  - Endothelial Growth Factors/*metabolism
MH  - Female
MH  - Humans
MH  - Lymphokines/*metabolism
MH  - Male
MH  - Microcirculation/pathology
MH  - Middle Aged
MH  - Neoplasm Invasiveness/pathology
MH  - *Neovascularization, Pathologic
MH  - Pancreas/blood supply/pathology/surgery
MH  - Pancreatectomy
MH  - Pancreatic Neoplasms/*blood supply/*metabolism/mortality/surgery
MH  - Prognosis
MH  - Survival Rate
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
EDAT- 2001/02/24 12:00
MHDA- 2001/03/07 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/03/07 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
AID - 10.1385/IJGC:28:1:31 [doi]
PST - ppublish
SO  - Int J Pancreatol. 2000 Aug;28(1):31-9. doi: 10.1385/IJGC:28:1:31.