PMID- 11278685
OWN - NLM
STAT- MEDLINE
DCOM- 20010628
LR  - 20220129
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 276
IP  - 18
DP  - 2001 May 4
TI  - Physical interaction with Yes-associated protein enhances p73 transcriptional 
      activity.
PG  - 15164-73
AB  - Specific protein-protein interactions are involved in a large number of cellular 
      processes and are mainly mediated by structurally and functionally defined 
      domains. Here we report that the nuclear phosphoprotein p73 can engage in a 
      physical association with the Yes-associated protein (YAP). This association 
      occurs under physiological conditions as shown by reciprocal 
      co-immunoprecipitation of complexes from lysates of P19 cells. The WW domain of 
      YAP and the PPPPY motif of p73 are directly involved in the association. 
      Furthermore, as required for ligands to group I WW domains, the terminal tyrosine 
      (Y) of the PPPPY motif of p73 was shown to be essential for the association with 
      YAP. Unlike p73alpha, p73beta, and p63alpha, which bind to YAP, the endogenous as 
      well as exogenously expressed wild-type p53 (wt-p53) and the p73gamma isoform do 
      not interact with YAP. Indeed, we documented that YAP interacts only with those 
      members of the p53 family that have a well conserved PPXY motif, a target 
      sequence for WW domains. Overexpression of YAP causes an increase of p73alpha 
      transcriptional activity. Differential interaction of YAP with members of the p53 
      family may provide a molecular explanation for their functional divergence in 
      signaling.
FAU - Strano, S
AU  - Strano S
AD  - Molecular Oncogenesis Laboratory, Regina Elena Cancer Institute, Rome 00158, 
      Italy.
FAU - Munarriz, E
AU  - Munarriz E
FAU - Rossi, M
AU  - Rossi M
FAU - Castagnoli, L
AU  - Castagnoli L
FAU - Shaul, Y
AU  - Shaul Y
FAU - Sacchi, A
AU  - Sacchi A
FAU - Oren, M
AU  - Oren M
FAU - Sudol, M
AU  - Sudol M
FAU - Cesareni, G
AU  - Cesareni G
FAU - Blandino, G
AU  - Blandino G
LA  - eng
GR  - 369/BI/TI_/Telethon/Italy
GR  - AR45626/AR/NIAMS NIH HHS/United States
GR  - CA45757/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20010124
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (TP73 protein, human)
RN  - 0 (Transcription Factors)
RN  - 0 (Tumor Protein p73)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 0 (YAP-Signaling Proteins)
RN  - 0 (YAP1 protein, human)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Carrier Proteins/*metabolism
MH  - Cell Line
MH  - DNA Primers
MH  - DNA-Binding Proteins/chemistry/genetics/*metabolism
MH  - Fluorescent Antibody Technique, Indirect
MH  - Genes, Tumor Suppressor
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Luminescent Proteins/genetics
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/chemistry/genetics/*metabolism
MH  - Phosphoproteins/*metabolism
MH  - Point Mutation
MH  - Protein Binding
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Signal Transduction
MH  - Transcription Factors
MH  - *Transcription, Genetic
MH  - Tumor Protein p73
MH  - Tumor Suppressor Proteins
MH  - YAP-Signaling Proteins
EDAT- 2001/03/30 10:00
MHDA- 2001/06/29 10:01
CRDT- 2001/03/30 10:00
PHST- 2001/03/30 10:00 [pubmed]
PHST- 2001/06/29 10:01 [medline]
PHST- 2001/03/30 10:00 [entrez]
AID - S0021-9258(19)56811-9 [pii]
AID - 10.1074/jbc.M010484200 [doi]
PST - ppublish
SO  - J Biol Chem. 2001 May 4;276(18):15164-73. doi: 10.1074/jbc.M010484200. Epub 2001 
      Jan 24.