PMID- 11518729
OWN - NLM
STAT- MEDLINE
DCOM- 20010920
LR  - 20220225
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 108
IP  - 4
DP  - 2001 Aug
TI  - Alpha4-integrin-VCAM-1 binding mediates G protein-independent capture of 
      encephalitogenic T cell blasts to CNS white matter microvessels.
PG  - 557-65
AB  - Direct in vivo evidence is still lacking for alpha4-integrin-mediated T cell 
      interaction with VCAM-1 on blood-brain barrier-endothelium in experimental 
      autoimmune encephalomyelitis (EAE). To investigate a possible 
      alpha4-integrin-mediated interaction of encephalitogenic T cell blasts with 
      VCAM-1 on the blood-brain barrier white matter endothelium in vivo, we have 
      developed a novel spinal cord window preparation that enabled us to directly 
      visualize CNS white matter microcirculation by intravital fluorescence 
      videomicroscopy. Our study provides the first in vivo evidence that 
      encephalitogenic T cell blasts interact with the spinal cord white matter 
      microvasculature without rolling and that alpha4-integrin mediates the G 
      protein-independent capture and subsequently the G protein-dependent adhesion 
      strengthening of T cell blasts to microvascular VCAM-1.
FAU - Vajkoczy, P
AU  - Vajkoczy P
AD  - Department of Neurosurgery, Klinikum Mannheim, Universitat Heidelberg, Mannheim, 
      Germany.
FAU - Laschinger, M
AU  - Laschinger M
FAU - Engelhardt, B
AU  - Engelhardt B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Antigens, CD)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 143198-26-9 (Integrin alpha4)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
CIN - J Clin Invest. 2001 Aug;108(4):517-9. PMID: 11518723
MH  - Animals
MH  - Antigens, CD/*metabolism
MH  - Autoimmune Diseases/immunology/*metabolism/pathology
MH  - Blood-Brain Barrier/*physiology
MH  - Capillaries/pathology
MH  - Cell Adhesion
MH  - Chemotaxis, Leukocyte/*physiology
MH  - Encephalomyelitis, Autoimmune, Experimental/immunology/*metabolism/pathology
MH  - Endothelium, Vascular/*metabolism/pathology
MH  - Female
MH  - GTP-Binding Proteins/antagonists & inhibitors/physiology
MH  - Integrin alpha4
MH  - Mice
MH  - Microcirculation
MH  - Microscopy, Fluorescence
MH  - Microscopy, Video
MH  - Nervous System Autoimmune Disease, Experimental
MH  - Protein Binding
MH  - Skin Window Technique
MH  - Spinal Cord/*blood supply/pathology
MH  - T-Lymphocyte Subsets/*metabolism/pathology
MH  - Vascular Cell Adhesion Molecule-1/*metabolism
MH  - Virulence Factors, Bordetella/genetics/pharmacology
PMC - PMC209399
EDAT- 2001/08/24 10:00
MHDA- 2001/09/21 10:01
PMCR- 2001/08/15
CRDT- 2001/08/24 10:00
PHST- 2001/08/24 10:00 [pubmed]
PHST- 2001/09/21 10:01 [medline]
PHST- 2001/08/24 10:00 [entrez]
PHST- 2001/08/15 00:00 [pmc-release]
AID - 12440 [pii]
AID - 10.1172/JCI12440 [doi]
PST - ppublish
SO  - J Clin Invest. 2001 Aug;108(4):557-65. doi: 10.1172/JCI12440.