PMID- 11884463
OWN - NLM
STAT- MEDLINE
DCOM- 20020415
LR  - 20220310
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 168
IP  - 6
DP  - 2002 Mar 15
TI  - Recruitment kinetics and composition of antibody-secreting cells within the 
      central nervous system following viral encephalomyelitis.
PG  - 2922-9
AB  - Infection by the neurotropic JHM strain of mouse hepatitis virus produces an 
      acute demyelinating encephalomyelitis. While cellular immunity initially 
      eliminates infectious virus, CNS viral persistence is predominantly controlled by 
      humoral immunity. To better understand the distinct phases of immune control 
      within the CNS, the kinetics of humoral immune responses were determined in 
      infected mice. Early during clearance of the JHM strain of mouse hepatitis virus, 
      only few virus-specific Ab-secreting cells (ASC) were detected in the periphery 
      or CNS, although mature B cells and ASC without viral specificity were recruited 
      into the CNS concomitant with T cells. Serum antiviral Ab and CNS virus-specific 
      ASC became prominent only during final elimination of infectious virus. 
      Virus-specific ASC peaked in lymphoid organs before the CNS, suggesting 
      peripheral B cell priming and maturation. Following elimination of infectious 
      virus, virus-specific ASC continued to increase within the CNS and then remained 
      stable during persistence, in contrast to declining T cell numbers. These data 
      comprise three novel findings. Rapid recruitment of B cells in the absence of 
      specific Ab secretion supports a potential Ab-independent effector function 
      involving lysis of virus-infected cells. Delayed recruitment relative to viral 
      clearance and subsequent maintenance of a stable CNS ASC population demonstrate 
      differential regulation of T and B lymphocytes within the infected CNS. This 
      supports a critical role of humoral immunity in regulating viral CNS persistence. 
      Lastly, altered antiviral ASC specificities following clearance of infectious 
      virus suggest ongoing recruitment of peripheral memory cells and/or local B cell 
      differentiation.
FAU - Tschen, Shuen-Ing
AU  - Tschen SI
AD  - Department of Molecular Microbiology and Immunology, Keck School of Medicine, 
      University of Southern California, Los Angeles, CA 90033, USA.
FAU - Bergmann, Cornelia C
AU  - Bergmann CC
FAU - Ramakrishna, Chandran
AU  - Ramakrishna C
FAU - Morales, Shawn
AU  - Morales S
FAU - Atkinson, Roscoe
AU  - Atkinson R
FAU - Stohlman, Stephen A
AU  - Stohlman SA
LA  - eng
GR  - AI47249/AI/NIAID NIH HHS/United States
GR  - NS18146/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/biosynthesis/blood
MH  - Antibody Specificity
MH  - Antibody-Producing Cells/*pathology/virology
MH  - Cell Movement/*immunology
MH  - Central Nervous System/*immunology/*pathology/virology
MH  - Coronavirus Infections/*immunology/pathology/virology
MH  - Encephalitis, Viral/*immunology/pathology/virology
MH  - Kinetics
MH  - Lymph Nodes/immunology/pathology/virology
MH  - Lymphocyte Count
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Murine hepatitis virus/*immunology/pathogenicity
MH  - Neck
MH  - Plasma Cells/immunology/pathology/virology
MH  - Species Specificity
MH  - Tumor Cells, Cultured
EDAT- 2002/03/09 10:00
MHDA- 2002/04/16 10:01
CRDT- 2002/03/09 10:00
PHST- 2002/03/09 10:00 [pubmed]
PHST- 2002/04/16 10:01 [medline]
PHST- 2002/03/09 10:00 [entrez]
AID - 10.4049/jimmunol.168.6.2922 [doi]
PST - ppublish
SO  - J Immunol. 2002 Mar 15;168(6):2922-9. doi: 10.4049/jimmunol.168.6.2922.