PMID- 12193690
OWN - NLM
STAT- MEDLINE
DCOM- 20020927
LR  - 20191210
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 169
IP  - 5
DP  - 2002 Sep 1
TI  - Shaping gene expression in activated and resting primary macrophages by IL-10.
PG  - 2253-63
AB  - IL-10 regulates inflammation by reducing cytokine and chemokine production from 
      activated macrophages. We performed microarray experiments to identify possible 
      effector molecules of IL-10 and to investigate the global effect of IL-10 on the 
      transcriptional response induced in LPS-activated macrophages. To exclude 
      background effects of endogenous IL-10, macrophages from IL-10-deficient mice 
      were used. IL-10 up-regulated expression of a small number of genes (26 and 37 
      after 45 min and 3 h, respectively), including newly identified and previously 
      documented targets such as suppressor of cytokine signaling-3 and IL-1 receptor 
      antagonist. However, the activation program triggered by LPS was profoundly 
      affected by IL-10. IL-10 repressed 62 and further increased 15 of 259 LPS-induced 
      genes. For all genes examined, the effects of IL-10 were determined to be 
      STAT3-dependent. These results suggest that IL-10 regulates STAT3-dependent 
      pathways that selectively target a broad component of LPS-induced genes at the 
      mRNA level.
FAU - Lang, Roland
AU  - Lang R
AD  - Department of Infectious Diseases, St. Jude Children's Research Hospital, 
      Memphis, TN 38015, USA.
FAU - Patel, Divyen
AU  - Patel D
FAU - Morris, John J
AU  - Morris JJ
FAU - Rutschman, Robert L
AU  - Rutschman RL
FAU - Murray, Peter J
AU  - Murray PJ
LA  - eng
GR  - P30 CA 21765/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Validation Study
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, Interleukin-4)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (Stat3 protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - EC 3.5.3.1 (Arginase)
SB  - IM
MH  - Animals
MH  - Arginase/biosynthesis
MH  - Bone Marrow Cells/cytology/enzymology/immunology/metabolism
MH  - DNA-Binding Proteins/deficiency/genetics/physiology
MH  - Down-Regulation/genetics/immunology
MH  - Gene Expression Regulation/*immunology
MH  - Interleukin-10/antagonists & inhibitors/deficiency/genetics/*physiology
MH  - Interleukin-4/physiology
MH  - Interphase/genetics/immunology
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophage Activation/*genetics/immunology
MH  - Macrophages/cytology/enzymology/*immunology/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Oligonucleotide Array Sequence Analysis/methods
MH  - Receptors, Interleukin-4/biosynthesis
MH  - STAT3 Transcription Factor
MH  - Signal Transduction/genetics/immunology
MH  - Trans-Activators/deficiency/genetics/physiology
MH  - Up-Regulation/genetics/immunology
EDAT- 2002/08/24 10:00
MHDA- 2002/09/28 04:00
CRDT- 2002/08/24 10:00
PHST- 2002/08/24 10:00 [pubmed]
PHST- 2002/09/28 04:00 [medline]
PHST- 2002/08/24 10:00 [entrez]
AID - 10.4049/jimmunol.169.5.2253 [doi]
PST - ppublish
SO  - J Immunol. 2002 Sep 1;169(5):2253-63. doi: 10.4049/jimmunol.169.5.2253.