PMID- 12221133
OWN - NLM
STAT- MEDLINE
DCOM- 20030312
LR  - 20181113
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 13
IP  - 9
DP  - 2002 Sep
TI  - Membrane trafficking of heterotrimeric G proteins via the endoplasmic reticulum 
      and Golgi.
PG  - 3294-302
AB  - Membrane targeting of G-protein alphabetagamma heterotrimers was investigated in 
      live cells by use of Galpha and Ggamma subunits tagged with spectral mutants of 
      green fluorescent protein. Unlike Ras proteins, Gbetagamma contains a single 
      targeting signal, the CAAX motif, which directed the dimer to the endoplasmic 
      reticulum. Endomembrane localization of farnesylated Ggamma(1), but not 
      geranylgeranylated Ggamma(2), required carboxyl methylation. Targeting of the 
      heterotrimer to the plasma membrane (PM) required coexpression of all three 
      subunits, combining the CAAX motif of Ggamma with the fatty acyl modifications of 
      Galpha. Galpha associated with Gbetagamma on the Golgi and palmitoylation of 
      Galpha was required for translocation of the heterotrimer to the PM. Thus, two 
      separate signals, analogous to the dual-signal targeting mechanism of Ras 
      proteins, cooperate to target heterotrimeric G proteins to the PM via the 
      endomembrane.
FAU - Michaelson, David
AU  - Michaelson D
AD  - Department of Medicine, Cell Biology and Pharmacology, NYU School of Medicine, 
      10016, USA.
FAU - Ahearn, Ian
AU  - Ahearn I
FAU - Bergo, Martin
AU  - Bergo M
FAU - Young, Stephen
AU  - Young S
FAU - Philips, Mark
AU  - Philips M
LA  - eng
GR  - CA-09161/CA/NCI NIH HHS/United States
GR  - T32 CA009161/CA/NCI NIH HHS/United States
GR  - M01 RR000096/RR/NCRR NIH HHS/United States
GR  - GM-55279/GM/NIGMS NIH HHS/United States
GR  - R29 AI036224/AI/NIAID NIH HHS/United States
GR  - R01 GM055279/GM/NIGMS NIH HHS/United States
GR  - AI-36224/AI/NIAID NIH HHS/United States
GR  - R01 AI036224/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (Luminescent Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Motifs
MH  - Animals
MH  - Biological Transport
MH  - COS Cells
MH  - Cell Line
MH  - Cell Membrane/metabolism
MH  - Dogs
MH  - Endoplasmic Reticulum/*metabolism
MH  - GTP-Binding Proteins/*metabolism
MH  - Golgi Apparatus/*metabolism
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Luminescent Proteins/metabolism
MH  - Microscopy, Fluorescence
MH  - Models, Biological
MH  - Plasmids/metabolism
MH  - Protein Binding
MH  - Protein Prenylation
MH  - Protein Structure, Tertiary
MH  - Recombinant Fusion Proteins/metabolism
MH  - Signal Transduction
PMC - PMC124159
EDAT- 2002/09/11 10:00
MHDA- 2003/03/13 04:00
PMCR- 2002/11/01
CRDT- 2002/09/11 10:00
PHST- 2002/09/11 10:00 [pubmed]
PHST- 2003/03/13 04:00 [medline]
PHST- 2002/09/11 10:00 [entrez]
PHST- 2002/11/01 00:00 [pmc-release]
AID - 1960 [pii]
AID - 10.1091/mbc.e02-02-0095 [doi]
PST - ppublish
SO  - Mol Biol Cell. 2002 Sep;13(9):3294-302. doi: 10.1091/mbc.e02-02-0095.