PMID- 12231631
OWN - NLM
STAT- MEDLINE
DCOM- 20021017
LR  - 20181113
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 16
IP  - 18
DP  - 2002 Sep 15
TI  - Synapsis-dependent and -independent mechanisms stabilize homolog pairing during 
      meiotic prophase in C. elegans.
PG  - 2428-42
AB  - Analysis of Caenorhabditis elegans syp-1 mutants reveals that both 
      synapsis-dependent and -independent mechanisms contribute to stable, productive 
      alignment of homologous chromosomes during meiotic prophase. Early prophase 
      nuclei undergo normal reorganization in syp-1 mutants, and chromosomes initially 
      pair. However, the polarized nuclear organization characteristic of early 
      prophase persists for a prolonged period, and homologs dissociate prematurely; 
      furthermore, the synaptonemal complex (SC) is absent. The predicted structure of 
      SYP-1, its localization at the interface between intimately paired, 
      lengthwise-aligned pachytene homologs, and its kinetics of localization with 
      chromosomes indicate that SYP-1 is an SC structural component. A severe reduction 
      in crossing over together with evidence for accumulated recombination 
      intermediates in syp-1 mutants indicate that initial pairing is not sufficient 
      for completion of exchange and implicates the SC in promoting crossover 
      recombination. Persistence of polarized nuclear organization in syp-1 mutants 
      suggests that SC polymerization may provide a motive force or signal that drives 
      redispersal of chromosomes. Whereas our analysis suggests that the SC is required 
      to stabilize pairing along the entire lengths of chromosomes, striking 
      differences in peak pairing levels for opposite ends of chromosomes in syp-1 
      mutants reveal the existence of an additional mechanism that can promote local 
      stabilization of pairing, independent of synapsis.
FAU - MacQueen, Amy J
AU  - MacQueen AJ
AD  - Department of Developmental Biology, Stanford University School of Medicine, 
      Stanford, California 94305-5329, USA.
FAU - Colaiacovo, Monica P
AU  - Colaiacovo MP
FAU - McDonald, Kent
AU  - McDonald K
FAU - Villeneuve, Anne M
AU  - Villeneuve AM
LA  - eng
GR  - F32 HD041329/HD/NICHD NIH HHS/United States
GR  - R01 GM053804/GM/NIGMS NIH HHS/United States
GR  - F32HD41329/HD/NICHD NIH HHS/United States
GR  - R01GM53804/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (HIM-3 protein, C elegans)
RN  - 0 (Helminth Proteins)
RN  - EC 3.1.- (Endodeoxyribonucleases)
RN  - EC 3.1.- (Esterases)
RN  - EC 3.1.- (meiotic recombination protein SPO11)
SB  - IM
MH  - Animals
MH  - Caenorhabditis elegans/*cytology/*genetics/metabolism
MH  - Caenorhabditis elegans Proteins/genetics/metabolism
MH  - Chromosome Pairing/*genetics
MH  - Endodeoxyribonucleases
MH  - Esterases/genetics/metabolism
MH  - Female
MH  - Genes, Helminth
MH  - Helminth Proteins/genetics/metabolism
MH  - Male
MH  - Meiosis/*genetics
MH  - Microscopy, Electron
MH  - Mutation
MH  - Prophase/genetics
MH  - Synaptonemal Complex/genetics
PMC - PMC187442
EDAT- 2002/09/17 10:00
MHDA- 2002/10/18 04:00
PMCR- 2003/03/15
CRDT- 2002/09/17 10:00
PHST- 2002/09/17 10:00 [pubmed]
PHST- 2002/10/18 04:00 [medline]
PHST- 2002/09/17 10:00 [entrez]
PHST- 2003/03/15 00:00 [pmc-release]
AID - 10.1101/gad.1011602 [doi]
PST - ppublish
SO  - Genes Dev. 2002 Sep 15;16(18):2428-42. doi: 10.1101/gad.1011602.