PMID- 1260762
OWN - NLM
STAT- MEDLINE
DCOM- 19760706
LR  - 20131121
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 36
IP  - 4
DP  - 1976 Apr
TI  - X-radiation-induced transformation in a C3H mouse embryo-derived cell line.
PG  - 1367-74
AB  - Reproducible X-ray-induced oncogenic transformation has been demonstrated in an 
      established cell line of mouse embryo fibroblasts. Cells derived from transformed 
      foci formed malignant tumors when injected into syngeneic hosts. An exponential 
      increase in the number of transformants per viable cell occurred with doses of up 
      to 400 rads of X-radiation. The transformation frequency in exponentially growing 
      cultures remained constant at 2.3 x 10(-3) following doses of 400 to 1500 rads. 
      There was little change in survival following X-ray doses up to 300 rads. Doses 
      greater than 300 rads were associated with an exponential decline in survival; 
      the Do for the survival curve was 175 rads. Transformation frequency varied with 
      changes in the number of viable cells seeded per dish. There was about a 10-fold 
      decline in the transformation frequency when the number of cells was increased 
      from 400 to 1000 viable cells/100-mm Petri dish. Below this density range there 
      was little change in transformation frequency. The presence of lethally 
      preir-radiated cells was not associated with an enhancement of transformation in 
      irradiated cells or with the induction of transformation in unirradiated cell 
      cultures. Amphotericin B (Fungizone) inhibited the appearance of transformants 
      when added to the culture medium within 2 to 3 weeks after initiation of the 
      experiment.
FAU - Terzaghi, M
AU  - Terzaghi M
FAU - Little, J B
AU  - Little JB
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 7XU7A7DROE (Amphotericin B)
SB  - IM
MH  - Amphotericin B/pharmacology
MH  - Animals
MH  - Cell Line
MH  - Cell Survival/radiation effects
MH  - *Cell Transformation, Neoplastic/drug effects/pathology
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Radiation
MH  - Fibroblasts/radiation effects
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Neoplasms, Experimental/etiology
MH  - *Radiation Effects
MH  - X-Rays
EDAT- 1976/04/01 00:00
MHDA- 1976/04/01 00:01
CRDT- 1976/04/01 00:00
PHST- 1976/04/01 00:00 [pubmed]
PHST- 1976/04/01 00:01 [medline]
PHST- 1976/04/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1976 Apr;36(4):1367-74.