PMID- 12865297
OWN - NLM
STAT- MEDLINE
DCOM- 20030808
LR  - 20250214
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 17
IP  - 14
DP  - 2003 Jul 15
TI  - Canonical Wnt signals are essential for homeostasis of the intestinal epithelium.
PG  - 1709-13
AB  - To assess the critical role of Wnt signals in intestinal crypts, we generated 
      transgenic mice ectopically expressing Dickkopf1 (Dkk1), a secreted Wnt 
      inhibitor. We find that epithelial proliferation is greatly reduced 
      coincidentally with the loss of crypts. Although enterocyte differentiation 
      appears unaffected, secretory cell lineages are largely absent. Disrupted 
      intestinal homeostasis is reflected by an absence of nuclear beta-catenin, 
      inhibition of c-myc expression, and subsequent up-regulation of p21CIP1/WAF1. 
      Thus, our data are the first to establish a direct requirement for Wnt ligands in 
      driving proliferation in the intestinal epithelium, and also define an unexpected 
      role for Wnts in controlling secretory cell differentiation.
FAU - Pinto, Daniel
AU  - Pinto D
AD  - Netherlands Institute for Developmental Biology, Hubrecht Laboratory, Uppsalalaan 
      8, 3584 CT Utrecht, The Netherlands.
FAU - Gregorieff, Alex
AU  - Gregorieff A
FAU - Begthel, Harry
AU  - Begthel H
FAU - Clevers, Hans
AU  - Clevers H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (CTNNB1 protein, mouse)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Dkk1 protein, mouse)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Wnt Proteins)
RN  - 0 (Zebrafish Proteins)
RN  - 0 (beta Catenin)
RN  - 0 (dkk1b protein, zebrafish)
SB  - IM
MH  - Animals
MH  - Cell Division/physiology
MH  - Cytoskeletal Proteins/metabolism
MH  - Homeostasis/*physiology
MH  - Intercellular Signaling Peptides and Proteins
MH  - Intestinal Mucosa/*metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Proteins/metabolism
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Signal Transduction/*physiology
MH  - Trans-Activators/metabolism
MH  - Wnt Proteins
MH  - *Zebrafish Proteins
MH  - beta Catenin
PMC - PMC196179
EDAT- 2003/07/17 05:00
MHDA- 2003/08/09 05:00
PMCR- 2004/01/15
CRDT- 2003/07/17 05:00
PHST- 2003/07/17 05:00 [pubmed]
PHST- 2003/08/09 05:00 [medline]
PHST- 2003/07/17 05:00 [entrez]
PHST- 2004/01/15 00:00 [pmc-release]
AID - 17/14/1709 [pii]
AID - 0171709 [pii]
AID - 10.1101/gad.267103 [doi]
PST - ppublish
SO  - Genes Dev. 2003 Jul 15;17(14):1709-13. doi: 10.1101/gad.267103.