PMID- 12944921
OWN - NLM
STAT- MEDLINE
DCOM- 20030922
LR  - 20121115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 22
IP  - 36
DP  - 2003 Aug 28
TI  - Use of adeno-associated viral vector for delivery of small interfering RNA.
PG  - 5712-5
AB  - Post-transcriptional gene silencing by small interfering RNAs (siRNAs) is rapidly 
      becoming a powerful tool for genetic analysis of mammalian cells. Delivery of 
      siRNA into mammalian cells is usually achieved via the transfection of 
      double-stranded oligonucleotides or plasmids encoding RNA polymerase III 
      promoter-driven small hairpin RNA. Recently, retroviral vectors have been used 
      for siRNA delivery, which overcome the problem of poor transfection efficiency 
      seen with the plasmid-based systems. However, retroviral vectors have several 
      limitations, such as the need for active cell division for gene transduction, 
      oncogenic potential, low titers and gene silencing. In this report, we have 
      adapted a commercially available adenoassociated virus (AAV) vector for siRNA 
      delivery into mammalian cells. We demonstrate the ability of this modified vector 
      to deliver efficiently siRNA into HeLa S3 cells and downregulate p53 and caspase 
      8 expression. Our results suggest that AAV-based vectors are efficient vectors 
      for the delivery of siRNA into mammalian cells. Based on the known ability of 
      these vectors to infect both dividing and nondividing cells, their use as a 
      therapeutic tool for the delivery of siRNA deserves further study.
FAU - Tomar, Raghuvir S
AU  - Tomar RS
AD  - Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern 
      Medical Center, Dallas, TX 75390-8593, USA.
FAU - Matta, Hittu
AU  - Matta H
FAU - Chaudhary, Preet M
AU  - Chaudhary PM
LA  - eng
GR  - P50-CA70907/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (RNA, Small Interfering)
RN  - EC 3.4.22.- (Caspase 8)
RN  - EC 3.4.22.- (Caspase 9)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Caspase 8
MH  - Caspase 9
MH  - Caspases/genetics
MH  - Dependovirus/*genetics
MH  - Genes, p53
MH  - *Genetic Therapy
MH  - *Genetic Vectors
MH  - RNA, Small Interfering/*administration & dosage
EDAT- 2003/08/29 05:00
MHDA- 2003/09/23 05:00
CRDT- 2003/08/29 05:00
PHST- 2003/08/29 05:00 [pubmed]
PHST- 2003/09/23 05:00 [medline]
PHST- 2003/08/29 05:00 [entrez]
AID - 1206733 [pii]
AID - 10.1038/sj.onc.1206733 [doi]
PST - ppublish
SO  - Oncogene. 2003 Aug 28;22(36):5712-5. doi: 10.1038/sj.onc.1206733.