PMID- 1359541
OWN - NLM
STAT- MEDLINE
DCOM- 19921223
LR  - 20220316
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 89
IP  - 22
DP  - 1992 Nov 15
TI  - Expression of the neu protooncogene in the mammary epithelium of transgenic mice 
      induces metastatic disease.
PG  - 10578-82
AB  - Overexpression and amplification of the neu (c-erbB2, ERBB2) protooncogene have 
      been implicated in the development of aggressive human breast cancer. To directly 
      assess the effect of mammary gland-specific expression of the neu protooncogene, 
      transgenic mice carrying unactivated neu under the transcriptional control of the 
      mouse mammary tumor virus promoter/enhancer were established. By contrast to the 
      rapid tumor progression observed in several transgenic strains carrying the 
      activated neu transgene, expression of unactivated neu in the mammary epithelium 
      resulted in the development of focal mammary tumors after long latency. The 
      majority of the mammary tumors analyzed expressed elevated levels of neu-encoded 
      mRNA and protein. Overexpression of neu in the mammary tumors was also associated 
      with elevated neu intrinsic tyrosine kinase activity and the de novo tyrosine 
      phosphorylation of several cellular proteins. Interestingly, many of the 
      tumor-bearing transgenic mice developed secondary metastatic tumors in the lung. 
      These observations suggest that overexpression of the unactivated neu protein can 
      induce metastatic disease after long latency.
FAU - Guy, C T
AU  - Guy CT
AD  - Institute for Molecular Biology and Biotechnology, McMaster University, Hamilton, 
      Ontario, Canada.
FAU - Webster, M A
AU  - Webster MA
FAU - Schaller, M
AU  - Schaller M
FAU - Parsons, T J
AU  - Parsons TJ
FAU - Cardiff, R D
AU  - Cardiff RD
FAU - Muller, W J
AU  - Muller WJ
LA  - eng
GR  - R01-CA S4285/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
GS  - ERBB2
GS  - c-erbB2
GS  - neu
MH  - Animals
MH  - Epithelium/pathology/physiology
MH  - Female
MH  - Kinetics
MH  - Male
MH  - Mammary Glands, Animal/pathology/*physiology
MH  - Mammary Neoplasms, Experimental/*genetics/*pathology
MH  - Mice
MH  - Mice, Transgenic
MH  - Neoplasm Metastasis/*genetics/pathology
MH  - Proto-Oncogene Proteins/*genetics
MH  - *Proto-Oncogenes
MH  - Receptor, ErbB-2
PMC - PMC50384
EDAT- 1992/11/15 00:00
MHDA- 1992/11/15 00:01
PMCR- 1993/05/15
CRDT- 1992/11/15 00:00
PHST- 1992/11/15 00:00 [pubmed]
PHST- 1992/11/15 00:01 [medline]
PHST- 1992/11/15 00:00 [entrez]
PHST- 1993/05/15 00:00 [pmc-release]
AID - 10.1073/pnas.89.22.10578 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10578-82. doi: 
      10.1073/pnas.89.22.10578.