PMID- 1381261
OWN - NLM
STAT- MEDLINE
DCOM- 19921007
LR  - 20190613
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 576
IP  - 2
DP  - 1992 Apr 3
TI  - TIMP-2 reduces proteolytic opening of blood-brain barrier by type IV collagenase.
PG  - 203-7
AB  - Intracerebral hemorrhage occurs in tumors, stroke and head trauma. Proteolysis of 
      the extracellular matrix around cerebral capillaries by naturally occurring 
      mammalian 72-kDa type IV collagenase may initiate this pathologic event. To 
      investigate this hypothesis adult rats underwent intracerebral injection of type 
      IV collagenase purified from human melanoma cells. Histologically, at 4 h there 
      was perivascular cellular infiltration with hemorrhage, and by 24 h there was 
      infarction with necrosis, edema and hemorrhage. Ultrastructurally, the basal 
      lamina of endothelial cells was disrupted at 2 h. Brain uptake of [14C]dextran 
      and [3H]sucrose increased after intracerebral injection of type IV collagenase 
      compared to controls (P less than 0.0001). Tissue inhibitor of 
      metalloproteinase-2 (TIMP-2) reduced the tracer uptake (P less than 0.02). 
      Metalloproteinase inhibitors reduce extracellular matrix proteolysis and protect 
      the blood-brain barrier.
FAU - Rosenberg, G A
AU  - Rosenberg GA
AD  - Department of Neurology, University of New Mexico School of Medicine, Albuquerque 
      87131-5281.
FAU - Kornfeld, M
AU  - Kornfeld M
FAU - Estrada, E
AU  - Estrada E
FAU - Kelley, R O
AU  - Kelley RO
FAU - Liotta, L A
AU  - Liotta LA
FAU - Stetler-Stevenson, W G
AU  - Stetler-Stevenson WG
LA  - eng
GR  - R01-NS21169/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Dextrans)
RN  - 0 (Neoplasm Proteins)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - 57-50-1 (Sucrose)
RN  - EC 3.4.24.3 (Microbial Collagenase)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Biological Transport/drug effects
MH  - Blood-Brain Barrier/*drug effects
MH  - Brain/drug effects/metabolism/*pathology
MH  - Capillaries/drug effects/pathology/ultrastructure
MH  - Cerebral Ventricles/drug effects/*physiology
MH  - Cerebrovascular Circulation/*drug effects
MH  - Dextrans/metabolism
MH  - Humans
MH  - Injections, Intraventricular
MH  - Male
MH  - Microbial Collagenase/administration & dosage/antagonists & inhibitors/*toxicity
MH  - Neoplasm Proteins/administration & dosage/*pharmacology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Sucrose/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-2
EDAT- 1992/04/03 00:00
MHDA- 1992/04/03 00:01
CRDT- 1992/04/03 00:00
PHST- 1992/04/03 00:00 [pubmed]
PHST- 1992/04/03 00:01 [medline]
PHST- 1992/04/03 00:00 [entrez]
AID - 0006-8993(92)90681-X [pii]
AID - 10.1016/0006-8993(92)90681-x [doi]
PST - ppublish
SO  - Brain Res. 1992 Apr 3;576(2):203-7. doi: 10.1016/0006-8993(92)90681-x.