PMID- 14647305
OWN - NLM
STAT- MEDLINE
DCOM- 20040805
LR  - 20201212
IS  - 1087-0156 (Print)
IS  - 1087-0156 (Linking)
VI  - 22
IP  - 1
DP  - 2004 Jan
TI  - Systemic tumor targeting and killing by Sindbis viral vectors.
PG  - 70-7
AB  - Successful cancer gene therapy requires a vector that systemically and 
      specifically targets tumor cells throughout the body. Although several vectors 
      have been developed to express cytotoxic genes via tumor-specific promoters or to 
      selectively replicate in tumor cells, most are taken up and expressed by just a 
      few targeted tumor cells. By contrast, we show here that blood-borne Sindbis 
      viral vectors systemically and specifically infect tumor cells. A single 
      intraperitoneal treatment allows the vectors to target most tumor cells, as 
      demonstrated by immunohistochemistry, without infecting normal cells. Further, 
      Sindbis infection is sufficient to induce complete tumor regression. We 
      demonstrate systemic vector targeting of tumors growing subcutaneously, 
      intrapancreatically, intraperitoneally and in the lungs. The vectors can also 
      target syngeneic and spontaneous tumors in immune-competent mice. We document the 
      anti-tumor specificity of a vector that systemically targets and eradicates tumor 
      cells throughout the body without adverse effects.
FAU - Tseng, Jen-Chieh
AU  - Tseng JC
AD  - New York University Gene Therapy Center, NYU Cancer Institute, NYU School of 
      Medicine, 550 First Avenue, New York, New York 10016, USA.
FAU - Levin, Brandi
AU  - Levin B
FAU - Hurtado, Alicia
AU  - Hurtado A
FAU - Yee, Herman
AU  - Yee H
FAU - Perez de Castro, Ignacio
AU  - Perez de Castro I
FAU - Jimenez, Maria
AU  - Jimenez M
FAU - Shamamian, Peter
AU  - Shamamian P
FAU - Jin, Ruzhong
AU  - Jin R
FAU - Novick, Richard P
AU  - Novick RP
FAU - Pellicer, Angel
AU  - Pellicer A
FAU - Meruelo, Daniel
AU  - Meruelo D
LA  - eng
GR  - CA22247/CA/NCI NIH HHS/United States
GR  - CA68498/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20031130
PL  - United States
TA  - Nat Biotechnol
JT  - Nature biotechnology
JID - 9604648
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Female
MH  - Genetic Therapy/*methods
MH  - Genetic Vectors
MH  - Immunohistochemistry
MH  - Luciferases/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, SCID
MH  - Neoplasm Metastasis
MH  - Neoplasm Transplantation
MH  - Neoplasms/*therapy
MH  - Sindbis Virus/*genetics
MH  - Time Factors
EDAT- 2003/12/03 05:00
MHDA- 2004/08/06 05:00
CRDT- 2003/12/03 05:00
PHST- 2003/10/01 00:00 [received]
PHST- 2003/10/29 00:00 [accepted]
PHST- 2003/12/03 05:00 [pubmed]
PHST- 2004/08/06 05:00 [medline]
PHST- 2003/12/03 05:00 [entrez]
AID - nbt917 [pii]
AID - 10.1038/nbt917 [doi]
PST - ppublish
SO  - Nat Biotechnol. 2004 Jan;22(1):70-7. doi: 10.1038/nbt917. Epub 2003 Nov 30.