PMID- 15110723 OWN - NLM STAT- MEDLINE DCOM- 20040602 LR - 20190523 IS - 0012-1606 (Print) IS - 1095-564X (Electronic) IS - 0012-1606 (Linking) VI - 269 IP - 2 DP - 2004 May 15 TI - Drawing lines in the sand: even skipped et al. and parasegment boundaries. PG - 609-22 AB - The pair-rule segmentation gene even skipped (eve) is required to activate engrailed stripes and to organize odd-numbered parasegments (PSs). The protein product Eve has been shown to be an active repressor of transcription, and recent models for Eve function suggest that activation of engrailed is indirect, but these models have not been fully tested. Here we identify the forkhead domain transcription factor Sloppy-paired as the key intermediate in the initial activation of engrailed by Eve in odd-numbered parasegments. We also analyze the roles of the transcription factors Runt and Odd-skipped in this process. Detailed analysis of engrailed and pair-rule gene expression in various mutant combinations shows how eve activates engrailed by repressing these engrailed repressors, and further indicates that mutual repression among pair-rule genes plays an important role in establishing parasegment boundaries. We present a new model of pair-rule gene function that explains the response of these boundaries to the relative levels of Eve and Fushi Tarazu. FAU - Jaynes, James B AU - Jaynes JB AD - Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, PA 19107, USA. J_Jaynes@mail.jci.tju.edu FAU - Fujioka, Miki AU - Fujioka M LA - eng GR - R01 GM050231/GM/NIGMS NIH HHS/United States GR - R01 GM050231-05A2/GM/NIGMS NIH HHS/United States GR - GM50231/GM/NIGMS NIH HHS/United States PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Dev Biol JT - Developmental biology JID - 0372762 RN - 0 (Blood Proteins) RN - 0 (C4a protein, mouse) RN - 0 (Complement C4) RN - 0 (DNA-Binding Proteins) RN - 0 (Drosophila Proteins) RN - 0 (En protein, Drosophila) RN - 0 (Homeodomain Proteins) RN - 0 (Nuclear Proteins) RN - 0 (Repressor Proteins) RN - 0 (Transcription Factors) RN - 0 (eve protein, Drosophila) RN - 0 (run protein, Drosophila) RN - 132191-97-0 (odd protein, Drosophila) SB - IM EIN - Dev Biol. 2004 Aug 1;272(1):277-8 MH - Animals MH - Blood Proteins/genetics/physiology MH - Complement C4 MH - DNA-Binding Proteins/physiology MH - Drosophila/*embryology/genetics MH - Drosophila Proteins/*physiology MH - *Gene Expression Regulation, Developmental MH - Homeodomain Proteins/genetics/*physiology MH - Nuclear Proteins MH - Repressor Proteins/physiology MH - Transcription Factors/genetics/*physiology PMC - PMC2709281 MID - NIHMS71672 EDAT- 2004/04/28 05:00 MHDA- 2004/06/03 05:00 PMCR- 2009/07/13 CRDT- 2004/04/28 05:00 PHST- 2004/02/26 00:00 [received] PHST- 2004/03/05 00:00 [accepted] PHST- 2004/04/28 05:00 [pubmed] PHST- 2004/06/03 05:00 [medline] PHST- 2004/04/28 05:00 [entrez] PHST- 2009/07/13 00:00 [pmc-release] AID - S0012160604001599 [pii] AID - 10.1016/j.ydbio.2004.03.001 [doi] PST - ppublish SO - Dev Biol. 2004 May 15;269(2):609-22. doi: 10.1016/j.ydbio.2004.03.001.