PMID- 15213235
OWN - NLM
STAT- MEDLINE
DCOM- 20041006
LR  - 20210206
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 279
IP  - 35
DP  - 2004 Aug 27
TI  - DNA repair defects channel interstrand DNA cross-links into alternate 
      recombinational and error-prone repair pathways.
PG  - 36462-9
AB  - The repair of psoralen interstrand cross-links in the yeast Saccharomyces 
      cerevisiae involves the DNA repair groups nucleotide excision repair (NER), 
      homologous recombination (HR), and post-replication repair (PRR). In 
      repair-proficient yeast cells cross-links induce double-strand breaks, in an 
      NER-dependent process; the double-strand breaks are then repaired by HR. An 
      alternate error-prone repair pathway generates mutations at cross-link sites. We 
      have characterized the repair of plasmid molecules carrying a single psoralen 
      cross-link, psoralen monoadduct, or double-strand break in yeast cells with 
      deficiencies in NER, HR, or PRR genes, measuring the repair efficiencies and the 
      levels of gene conversions, crossing over, and mutations. Strains with 
      deficiencies in the NER genes RAD1, RAD3, RAD4, and RAD10 had low levels of 
      cross-link-induced recombination but higher mutation frequencies than 
      repair-proficient cells. Deletion of the HR genes RAD51, RAD52, RAD54, RAD55, and 
      RAD57 also decreased induced recombination and increased mutation frequencies 
      above those of NER-deficient yeast. Strains lacking the PRR genes RAD5, RAD6, and 
      RAD18 did not have any cross-link-induced mutations but showed increased levels 
      of recombination; rad5 and rad6 cells also had altered patterns of 
      cross-link-induced gene conversion in comparison with repair-proficient yeast. 
      Our observations suggest that psoralen cross-links can be repaired by three 
      pathways: an error-free recombinational pathway requiring NER and HR and two 
      PRR-dependent error-prone pathways, one NER-dependent and one NER-independent.
FAU - Saffran, Wilma A
AU  - Saffran WA
AD  - Department of Chemistry and Biochemistry, Queens College of the City University 
      of New York, 65-30 Kissena Boulevard, Flushing, NY 11367, USA. 
      Wilma_Saffran@qc.edu
FAU - Ahmed, Shaila
AU  - Ahmed S
FAU - Bellevue, Sherly
AU  - Bellevue S
FAU - Pereira, Gillian
AU  - Pereira G
FAU - Patrick, Teleka
AU  - Patrick T
FAU - Sanchez, Wendy
AU  - Sanchez W
FAU - Thomas, Sandra
AU  - Thomas S
FAU - Alberti, Marie
AU  - Alberti M
FAU - Hearst, John E
AU  - Hearst JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040622
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (DNA, Fungal)
RN  - 9007-49-2 (DNA)
RN  - KTZ7ZCN2EX (Ficusin)
SB  - IM
MH  - Alleles
MH  - Cross-Linking Reagents/*pharmacology
MH  - DNA/metabolism
MH  - DNA Damage
MH  - *DNA Repair
MH  - DNA, Fungal
MH  - Ficusin/*chemistry/metabolism
MH  - Models, Biological
MH  - Models, Genetic
MH  - Mutagenesis
MH  - Mutation
MH  - Phenotype
MH  - Plasmids/metabolism
MH  - *Recombination, Genetic
MH  - Saccharomyces cerevisiae/metabolism
EDAT- 2004/06/24 05:00
MHDA- 2004/10/07 09:00
CRDT- 2004/06/24 05:00
PHST- 2004/06/24 05:00 [pubmed]
PHST- 2004/10/07 09:00 [medline]
PHST- 2004/06/24 05:00 [entrez]
AID - S0021-9258(20)85889-X [pii]
AID - 10.1074/jbc.M402323200 [doi]
PST - ppublish
SO  - J Biol Chem. 2004 Aug 27;279(35):36462-9. doi: 10.1074/jbc.M402323200. Epub 2004 
      Jun 22.