PMID- 15240568
OWN - NLM
STAT- MEDLINE
DCOM- 20040826
LR  - 20220311
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 166
IP  - 1
DP  - 2004 Jul 5
TI  - SOX9 is an intestine crypt transcription factor, is regulated by the Wnt pathway, 
      and represses the CDX2 and MUC2 genes.
PG  - 37-47
AB  - TCF and SOX proteins belong to the high mobility group box transcription factor 
      family. Whereas TCFs, the transcriptional effectors of the Wnt pathway, have been 
      widely implicated in the development, homeostasis and disease of the intestine 
      epithelium, little is known about the function of the SOX proteins in this 
      tissue. Here, we identified SOX9 in a SOX expression screening in the mouse fetal 
      intestine. We report that the SOX9 protein is expressed in the intestinal 
      epithelium in a pattern characteristic of Wnt targets. We provide in vitro and in 
      vivo evidence that a bipartite beta-catenin/TCF4 transcription factor, the 
      effector of the Wnt signaling pathway, is required for SOX9 expression in 
      epithelial cells. Finally, in colon epithelium-derived cells, SOX9 
      transcriptionally represses the CDX2 and MUC2 genes, normally expressed in the 
      mature villus cells of the intestinal epithelium, and may therefore contribute to 
      the Wnt-dependent maintenance of a progenitor cell phenotype.
FAU - Blache, Philippe
AU  - Blache P
AD  - Institut de Genetique Humaine, CNRS UPR1142, 141 rue de la Cardonille, 34396 
      Montpellier, Cedex 5, France.
FAU - van de Wetering, Marc
AU  - van de Wetering M
FAU - Duluc, Isabelle
AU  - Duluc I
FAU - Domon, Claire
AU  - Domon C
FAU - Berta, Philippe
AU  - Berta P
FAU - Freund, Jean-Noel
AU  - Freund JN
FAU - Clevers, Hans
AU  - Clevers H
FAU - Jay, Philippe
AU  - Jay P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (CDX2 Transcription Factor)
RN  - 0 (CTNNB1 protein, human)
RN  - 0 (CTNNB1 protein, mouse)
RN  - 0 (Cdx2 protein, mouse)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (High Mobility Group Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (MUC2 protein, human)
RN  - 0 (Muc2 protein, mouse)
RN  - 0 (Mucin-2)
RN  - 0 (Mucins)
RN  - 0 (SOX9 Transcription Factor)
RN  - 0 (SOX9 protein, human)
RN  - 0 (Sox9 protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (beta Catenin)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - CDX2 Transcription Factor
MH  - Carcinoma/metabolism
MH  - Cell Differentiation
MH  - Cell Line, Tumor
MH  - Cell Nucleus/metabolism
MH  - Colonic Neoplasms/metabolism
MH  - Cytoskeletal Proteins/metabolism
MH  - DNA/metabolism
MH  - Epithelium/metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - High Mobility Group Proteins/*metabolism
MH  - Homeodomain Proteins/*metabolism
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Immunohistochemistry
MH  - Intestinal Mucosa/metabolism
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Mucin-2
MH  - Mucins/*metabolism
MH  - Neoplasm Transplantation
MH  - Phenotype
MH  - RNA/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - SOX9 Transcription Factor
MH  - Signal Transduction
MH  - Stem Cells/metabolism
MH  - Trans-Activators/metabolism
MH  - Transcription Factors/*metabolism
MH  - Transcription, Genetic
MH  - Transfection
MH  - beta Catenin
PMC - PMC2172132
EDAT- 2004/07/09 05:00
MHDA- 2004/08/27 05:00
PMCR- 2005/01/05
CRDT- 2004/07/09 05:00
PHST- 2004/07/09 05:00 [pubmed]
PHST- 2004/08/27 05:00 [medline]
PHST- 2004/07/09 05:00 [entrez]
PHST- 2005/01/05 00:00 [pmc-release]
AID - jcb.200311021 [pii]
AID - 200311021 [pii]
AID - 10.1083/jcb.200311021 [doi]
PST - ppublish
SO  - J Cell Biol. 2004 Jul 5;166(1):37-47. doi: 10.1083/jcb.200311021.