PMID- 1557402
OWN - NLM
STAT- MEDLINE
DCOM- 19920506
LR  - 20220223
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 89
IP  - 7
DP  - 1992 Apr 1
TI  - Structural alterations of the epidermal growth factor receptor gene in human 
      gliomas.
PG  - 2965-9
AB  - The epidermal growth factor receptor (EGFR) gene is amplified in 40% of malignant 
      gliomas, and the amplified genes are frequently rearranged. We have characterized 
      the genetic alterations associated with these rearrangements in five malignant 
      gliomas. In one tumor the rearrangement resulted in the deletion of most of the 
      extracytoplasmic domain of the receptor, resulting in a hybrid mRNA between new 
      sequences and the truncated EGFR sequence. The predicted amino acid sequence of 
      the protein from this tumor was remarkably similar to that described for several 
      viral erbB oncogenes. Four other tumors were noted to have internal deletions of 
      the EGFR gene. These rearrangements brought about in-frame deletions affecting 
      either of two cysteine-rich domains in the extracytoplasmic portion of the 
      molecule. The clonal nature of these alterations, and the fact that identical 
      alterations were seen in more than one tumor, suggests a role for these mutant 
      receptor proteins in tumorigenesis. Further, these studies document the existence 
      of tumor-specific cell surface molecules resulting from somatic mutation.
FAU - Wong, A J
AU  - Wong AJ
AD  - Oncology Center, Johns Hopkins University School of Medicine, Baltimore, MD 
      21231.
FAU - Ruppert, J M
AU  - Ruppert JM
FAU - Bigner, S H
AU  - Bigner SH
FAU - Grzeschik, C H
AU  - Grzeschik CH
FAU - Humphrey, P A
AU  - Humphrey PA
FAU - Bigner, D S
AU  - Bigner DS
FAU - Vogelstein, B
AU  - Vogelstein B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Chromosome Deletion
MH  - Cloning, Molecular
MH  - ErbB Receptors/*genetics
MH  - Gene Expression
MH  - Gene Rearrangement
MH  - *Genes, Neoplasm
MH  - Glioma/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides/chemistry
MH  - RNA, Messenger/genetics
MH  - Restriction Mapping
PMC - PMC48784
EDAT- 1992/04/01 00:00
MHDA- 1992/04/01 00:01
PMCR- 1992/10/01
CRDT- 1992/04/01 00:00
PHST- 1992/04/01 00:00 [pubmed]
PHST- 1992/04/01 00:01 [medline]
PHST- 1992/04/01 00:00 [entrez]
PHST- 1992/10/01 00:00 [pmc-release]
AID - 10.1073/pnas.89.7.2965 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2965-9. doi: 10.1073/pnas.89.7.2965.