PMID- 158571
OWN - NLM
STAT- MEDLINE
DCOM- 19791220
LR  - 20190902
IS  - 0367-8377 (Print)
IS  - 0367-8377 (Linking)
VI  - 14
IP  - 2
DP  - 1979 Aug
TI  - A lipophilic derivative of neocarzinostatin. A polymer conjugation of an 
      antitumor protein antibiotic.
PG  - 81-7
AB  - A lipophilic derivative of neocarzinostatin (NCS), an antitumor antibiotic, was 
      prepared by reaction with a synthetic water-soluble polymer, [(styrene)1 
      approximately 3-(maleic acid 4 approximately 7/anhydride 1)]. The reaction was 
      carried out at pH 8.6 for 3 h and aimed at modifying the two nonessential amino 
      groups (alpha-amino of Ala-1, epsilon-amino of Lys-20). The NCS-polystyrene 
      (SMANCS) was purified on a column of Sephadex G-100 in 0.05 M ammonium 
      bicarbonate and the main product was obtained as a single peak. The elemental 
      analysis showed an increased C and a decreased N content. U.v. and i.r. 
      absorption spectra for SMANCS showed the presence of styrene. SDS-acrylamide gel 
      electrophoresis at pH 8.5 and the decreased N content suggested a molecular 
      weight of about 25 000, indicating the numbers of polymers conjugated to be about 
      six units, two of which were found attached to the two amino groups. SMANCS was 
      soluble in organic solvents, in contrast to NCS, and in water. SMANCS exhibited 
      increased chemical and biological stability and appeared to possess similar in 
      vitro biological activity.
FAU - Maeda, H
AU  - Maeda H
FAU - Takeshita, J
AU  - Takeshita J
FAU - Kanamaru, R
AU  - Kanamaru R
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Int J Pept Protein Res
JT  - International journal of peptide and protein research
JID - 0330420
RN  - 0 (Amino Acids)
RN  - 0 (Antibiotics, Antineoplastic)
RN  - 0 (Polymers)
RN  - 9014-02-2 (Zinostatin)
SB  - IM
MH  - Amino Acids/analysis
MH  - *Antibiotics, Antineoplastic/*chemical synthesis
MH  - Chromatography, Gel
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Polymers
MH  - Zinostatin/analogs & derivatives/analysis/*chemical synthesis
EDAT- 1979/08/01 00:00
MHDA- 1979/08/01 00:01
CRDT- 1979/08/01 00:00
PHST- 1979/08/01 00:00 [pubmed]
PHST- 1979/08/01 00:01 [medline]
PHST- 1979/08/01 00:00 [entrez]
AID - 10.1111/j.1399-3011.1979.tb01730.x [doi]
PST - ppublish
SO  - Int J Pept Protein Res. 1979 Aug;14(2):81-7. doi: 
      10.1111/j.1399-3011.1979.tb01730.x.