PMID- 15936993
OWN - NLM
STAT- MEDLINE
DCOM- 20051003
LR  - 20211203
IS  - 1568-7864 (Print)
IS  - 1568-7856 (Linking)
VI  - 4
IP  - 7
DP  - 2005 Jul 12
TI  - The Artemis:DNA-PKcs endonuclease cleaves DNA loops, flaps, and gaps.
PG  - 845-51
AB  - In eukaryotic cells, nonhomologous DNA end joining (NHEJ) is a major pathway for 
      repair of double-strand DNA breaks (DSBs). Artemis and the 469kDa DNA-dependent 
      protein kinase (DNA-PKcs) together form a key nuclease for NHEJ in vertebrate 
      organisms. The structure-specific endonucleolytic activity of Artemis is 
      activated by binding to and phosphorylation by DNA-PKcs. We tested various DNA 
      structures in order to understand the range of structural features that are 
      recognized by the Artemis:DNA-PKcs complex. We find that all tested substrates 
      that contain single-to-double-strand transitions can be cleaved by the 
      Artemis:DNA-PKcs complex near the transition region. The cleaved substrates 
      include heterologous loops, stem-loops, flaps, and gapped substrates. Such 
      versatile activity on single-/double-strand transition regions is important in 
      understanding how reconstituted NHEJ systems that lack DNA polymerases can join 
      incompatible DNA ends and yet preserve 3' overhangs. Additionally, the 
      flexibility of the Artemis:DNA-PKcs nuclease may be important in removing 
      secondary structures that hinder processing of DNA ends during NHEJ.
FAU - Ma, Yunmei
AU  - Ma Y
AD  - Department of Pathology, Norris Comprehensive Cancer Center, Rm. 5428, University 
      of Southern California Keck School of Medicine, Los Angeles, CA 90089-9176, USA.
FAU - Schwarz, Klaus
AU  - Schwarz K
FAU - Lieber, Michael R
AU  - Lieber MR
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - DNA Repair (Amst)
JT  - DNA repair
JID - 101139138
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.11.1 (DNA-Activated Protein Kinase)
RN  - EC 2.7.11.1 (PRKDC protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 3.1.- (DCLRE1C protein, human)
RN  - EC 3.1.- (Endonucleases)
SB  - IM
MH  - DNA/*chemistry/*metabolism
MH  - DNA, Single-Stranded/chemistry
MH  - DNA-Activated Protein Kinase
MH  - DNA-Binding Proteins/chemistry/isolation & purification/*metabolism
MH  - Endonucleases
MH  - Humans
MH  - Nuclear Proteins/chemistry/isolation & purification/*metabolism
MH  - Nucleic Acid Conformation
MH  - Protein Serine-Threonine Kinases/chemistry/isolation & purification/*metabolism
MH  - Recombinant Proteins/chemistry/isolation & purification
MH  - Recombination, Genetic
MH  - Substrate Specificity
EDAT- 2005/06/07 09:00
MHDA- 2005/10/04 09:00
CRDT- 2005/06/07 09:00
PHST- 2005/03/26 00:00 [received]
PHST- 2005/03/30 00:00 [revised]
PHST- 2005/04/25 00:00 [accepted]
PHST- 2005/06/07 09:00 [pubmed]
PHST- 2005/10/04 09:00 [medline]
PHST- 2005/06/07 09:00 [entrez]
AID - S1568-7864(05)00097-2 [pii]
AID - 10.1016/j.dnarep.2005.04.013 [doi]
PST - ppublish
SO  - DNA Repair (Amst). 2005 Jul 12;4(7):845-51. doi: 10.1016/j.dnarep.2005.04.013.