PMID- 16083717
OWN - NLM
STAT- MEDLINE
DCOM- 20050907
LR  - 20061115
IS  - 0016-5085 (Print)
IS  - 0016-5085 (Linking)
VI  - 129
IP  - 2
DP  - 2005 Aug
TI  - Expression pattern of Wnt signaling components in the adult intestine.
PG  - 626-38
AB  - BACKGROUND & AIMS: In the intestine, the canonical Wnt signaling cascade plays a 
      crucial role in driving the proliferation of epithelial cells. Furthermore, 
      aberrant activation of Wnt signaling is strongly associated with the development 
      of colorectal cancer. Despite this evidence, little is known about the precise 
      identity and localization of Wnts and their downstream effectors in the adult 
      intestine. To address this issue, we examined the expression pattern of all Wnts, 
      Frizzleds (Fzs), low-density lipoprotein receptor-related proteins, Wnt 
      antagonists, and T-cell factors in the murine small intestine and colon and 
      adenomas. METHODS: Embryonic, postnatal, and adult intestinal samples were 
      subjected to in situ hybridization by using specific RNA probes for the various 
      genes tested. RESULTS: Our analysis showed high expression of several signaling 
      components (including Wnt-3, Wnt-6, Wnt-9b, Frizzled 4, Frizzled 6, Frizzled 7, 
      low-density lipoprotein receptor-related protein 5, and secreted Frizzled-related 
      protein 5) in crypt epithelial cells. We also detected Wnt-2b, Wnt-4, Wnt-5a, 
      Wnt-5b, Frizzled 4, and Frizzled 6 in differentiated epithelial and mesenchymal 
      cells of the small intestine and colon. Finally, several factors (Frizzled 4, 
      T-cell factor 1, lymphoid enhancer factor, Dickkopf 2, Dickkopf 3, and 
      Wnt-interacting factor) displayed differential expression in normal vs neoplastic 
      tissue. CONCLUSIONS: Our study predicts a much broader role for Wnt signaling in 
      gut development and homeostasis than was previously anticipated from available 
      genetic studies and identifies novel factors likely involved in promoting 
      canonical and noncanonical Wnt signals in the intestine.
FAU - Gregorieff, Alex
AU  - Gregorieff A
AD  - Netherlands Institute for Developmental Biology and Center for Biomedical 
      Genetics, Hubrecht Laboratory, Utrecht.
FAU - Pinto, Daniel
AU  - Pinto D
FAU - Begthel, Harry
AU  - Begthel H
FAU - Destree, Olivier
AU  - Destree O
FAU - Kielman, Menno
AU  - Kielman M
FAU - Clevers, Hans
AU  - Clevers H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Gastroenterology
JT  - Gastroenterology
JID - 0374630
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Wnt Proteins)
RN  - 0 (Zebrafish Proteins)
SB  - IM
MH  - Adult
MH  - Age Factors
MH  - Biopsy, Needle
MH  - Cell Differentiation
MH  - Female
MH  - Fetus/pathology
MH  - *Gene Expression Regulation, Developmental
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Infant, Newborn
MH  - Intercellular Signaling Peptides and Proteins/analysis/*genetics
MH  - Intestine, Large/embryology/*pathology
MH  - Intestine, Small/embryology/*pathology
MH  - Male
MH  - Sensitivity and Specificity
MH  - Signal Transduction
MH  - Tissue Culture Techniques
MH  - Wnt Proteins
MH  - Zebrafish Proteins/*genetics
EDAT- 2005/08/09 09:00
MHDA- 2005/09/08 09:00
CRDT- 2005/08/09 09:00
PHST- 2004/10/28 00:00 [received]
PHST- 2005/05/11 00:00 [accepted]
PHST- 2005/08/09 09:00 [pubmed]
PHST- 2005/09/08 09:00 [medline]
PHST- 2005/08/09 09:00 [entrez]
AID - S0016-5085(05)01105-4 [pii]
AID - 10.1016/j.gastro.2005.06.007 [doi]
PST - ppublish
SO  - Gastroenterology. 2005 Aug;129(2):626-38. doi: 10.1016/j.gastro.2005.06.007.