PMID- 16228010
OWN - NLM
STAT- MEDLINE
DCOM- 20060127
LR  - 20190613
IS  - 1465-7392 (Print)
IS  - 1465-7392 (Linking)
VI  - 7
IP  - 11
DP  - 2005 Nov
TI  - Drosophila Ric-8 regulates Galphai cortical localization to promote 
      Galphai-dependent planar orientation of the mitotic spindle during asymmetric 
      cell division.
PG  - 1083-90
AB  - Localization and activation of heterotrimeric G proteins have a crucial role 
      during asymmetric cell division. The asymmetric division of the Drosophila 
      sensory precursor cell (pl) is polarized along the antero-posterior axis by 
      Frizzled signalling and, during this division, activation of Galphai depends on 
      Partner of Inscuteable (Pins). We establish here that Ric-8, which belongs to a 
      family of guanine nucleotide-exchange factors for Galphai, regulates cortical 
      localization of the subunits Galphai and Gbeta13F. Ric-8, Galphai and Pins are 
      not necessary for the control of the anteroposterior orientation of the mitotic 
      spindle during pl cell division downstream of Frizzled signalling, but they are 
      required for maintainance of the spindle within the plane of the epithelium. On 
      the contrary, Frizzled signalling orients the spindle along the antero-posterior 
      axis but also tilts it along the apico-basal axis. Thus, Frizzled and 
      heterotrimeric G-protein signalling act in opposition to ensure that the spindle 
      aligns both in the plane of the epithelium and along the tissue polarity axis.
FAU - David, Nicolas B
AU  - David NB
AD  - Institute Curie, CNRS UMR 144, 26 rue d'Ulm, Paris 75248, France. 
      nicolas.david@curie.fr
FAU - Martin, Charlotte A
AU  - Martin CA
FAU - Segalen, Marion
AU  - Segalen M
FAU - Rosenfeld, Francois
AU  - Rosenfeld F
FAU - Schweisguth, Francois
AU  - Schweisguth F
FAU - Bellaiche, Yohanns
AU  - Bellaiche Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nat Cell Biol
JT  - Nature cell biology
JID - 100890575
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Frizzled Receptors)
RN  - 0 (GTP-Binding Protein beta Subunits)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (gbb protein, Drosophila)
RN  - 0 (ric8a protein, Drosophila)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.1 (GTP-Binding Protein alpha Subunits, Gi-Go)
SB  - IM
CIN - Nat Cell Biol. 2005 Nov;7(11):1047-9. PMID: 16385732
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Cell Cycle Proteins/metabolism
MH  - Cell Division/*physiology
MH  - Cell Polarity
MH  - Drosophila/genetics/*metabolism
MH  - Drosophila Proteins/metabolism/physiology
MH  - Frizzled Receptors/physiology
MH  - GTP-Binding Protein alpha Subunits, Gi-Go/*metabolism
MH  - GTP-Binding Protein beta Subunits/physiology
MH  - GTP-Binding Proteins/genetics/*metabolism
MH  - Guanine Nucleotide Exchange Factors/*physiology
MH  - Neurons, Afferent/*physiology
MH  - Spindle Apparatus/metabolism
MH  - Stem Cells/metabolism
MH  - Transforming Growth Factor beta/physiology
EDAT- 2005/10/18 09:00
MHDA- 2006/01/28 09:00
CRDT- 2005/10/18 09:00
PHST- 2005/05/23 00:00 [received]
PHST- 2005/09/22 00:00 [accepted]
PHST- 2005/10/18 09:00 [pubmed]
PHST- 2006/01/28 09:00 [medline]
PHST- 2005/10/18 09:00 [entrez]
AID - ncb1319 [pii]
AID - 10.1038/ncb1319 [doi]
PST - ppublish
SO  - Nat Cell Biol. 2005 Nov;7(11):1083-90. doi: 10.1038/ncb1319.