PMID- 16332960
OWN - NLM
STAT- MEDLINE
DCOM- 20060530
LR  - 20240314
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 102
IP  - 50
DP  - 2005 Dec 13
TI  - A WW domain protein TAZ is a critical coactivator for TBX5, a transcription 
      factor implicated in Holt-Oram syndrome.
PG  - 18034-9
AB  - The T-box transcription factor TBX5 plays essential roles in cardiac and limb 
      development. Various mutations in the TBX5 gene have been identified in patients 
      with Holt-Oram syndrome, which is characterized by congenital defects in the 
      heart and upper extremities. In this study, we identified a WW-domain-containing 
      transcriptional regulator TAZ as a potent TBX5 coactivator. TAZ directly 
      associates with TBX5 and markedly stimulates TBX5-dependent promoters by 
      interacting with the histone acetyltransferases p300 and PCAF. YAP, a TAZ-related 
      protein with conserved functional domains, also stimulates TBX5-dependent 
      transcription, possibly by forming a heterodimer with TAZ. TBX5 lacks a PY motif, 
      which mediates the association of other proteins with TAZ, and interacts with TAZ 
      through multiple domains including its carboxyl-terminal structure. Truncation 
      mutants of TBX5 identified in patients with Holt-Oram syndrome were markedly 
      impaired in their ability to associate with and be stimulated by TAZ. These 
      findings reveal key roles for TAZ and YAP in the control of TBX5-dependent 
      transcription and suggest the involvement of these coactivators in cardiac and 
      limb development.
FAU - Murakami, Masao
AU  - Murakami M
AD  - Department of Molecular Biology, University of Texas Southwestern Medical Center, 
      Dallas, 75390-9148, USA.
FAU - Nakagawa, Masayo
AU  - Nakagawa M
FAU - Olson, Eric N
AU  - Olson EN
FAU - Nakagawa, Osamu
AU  - Nakagawa O
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051206
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA Primers)
RN  - 0 (Proteins)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (T-box transcription factor 5)
RN  - 0 (Transcription Factors)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.- (TAFAZZIN protein, human)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
SB  - IM
MH  - Abnormalities, Multiple/*genetics
MH  - Acyltransferases
MH  - Animals
MH  - Blotting, Western
MH  - DNA Primers
MH  - Electrophoretic Mobility Shift Assay
MH  - Heart Defects, Congenital/*genetics
MH  - Histone Acetyltransferases
MH  - Humans
MH  - Luciferases
MH  - Mice
MH  - Plasmids/genetics
MH  - Proteins/*metabolism
MH  - RNA Interference
MH  - Syndrome
MH  - T-Box Domain Proteins/*metabolism
MH  - Transcription Factors/*metabolism
MH  - Transcriptional Activation/*genetics
PMC - PMC1312418
EDAT- 2005/12/08 09:00
MHDA- 2006/05/31 09:00
PMCR- 2006/06/13
CRDT- 2005/12/08 09:00
PHST- 2005/12/08 09:00 [pubmed]
PHST- 2006/05/31 09:00 [medline]
PHST- 2005/12/08 09:00 [entrez]
PHST- 2006/06/13 00:00 [pmc-release]
AID - 0509109102 [pii]
AID - 18034 [pii]
AID - 10.1073/pnas.0509109102 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):18034-9. doi: 
      10.1073/pnas.0509109102. Epub 2005 Dec 6.