PMID- 16342157
OWN - NLM
STAT- MEDLINE
DCOM- 20060323
LR  - 20220318
IS  - 0740-3194 (Print)
IS  - 0740-3194 (Linking)
VI  - 55
IP  - 1
DP  - 2006 Jan
TI  - In vivo magnetic resonance imaging of single cells in mouse brain with optical 
      validation.
PG  - 23-9
AB  - In the current work we demonstrate, for the first time, that single cells can be 
      detected in mouse brain in vivo using magnetic resonance imaging (MRI). Cells 
      were labeled with superparamagnetic iron oxide nanoparticles and injected into 
      the circulation of mice. Individual cells trapped within the microcirculation of 
      the brain could be visualized with high-resolution MRI using optimized MR 
      hardware and the fast imaging employing steady state acquisition (FIESTA) pulse 
      sequence on a 1.5 T clinical MRI scanner. Single cells appear as discrete signal 
      voids on MR images. Direct optical validation was provided by coregistering 
      signal voids on MRI with single cells visualized using high-resolution confocal 
      microscopy. This work demonstrates the sensitivity of MRI for detecting single 
      cells in small animals for a wide range of application from stem cell to cancer 
      cell tracking.
FAU - Heyn, Chris
AU  - Heyn C
AD  - Imaging Research Laboratories, Robarts Research Institute, London, Ontario, 
      Canada. cheyn@imaging.robarts.ca
FAU - Ronald, John A
AU  - Ronald JA
FAU - Mackenzie, Lisa T
AU  - Mackenzie LT
FAU - MacDonald, Ian C
AU  - MacDonald IC
FAU - Chambers, Ann F
AU  - Chambers AF
FAU - Rutt, Brian K
AU  - Rutt BK
FAU - Foster, Paula J
AU  - Foster PJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Magn Reson Med
JT  - Magnetic resonance in medicine
JID - 8505245
RN  - 0 (Contrast Media)
RN  - 0 (Dextrans)
RN  - 0 (Magnetite Nanoparticles)
RN  - 0 (Oxides)
RN  - E1UOL152H7 (Iron)
RN  - G6N3J05W84 (ferumoxides)
RN  - XM0M87F357 (Ferrosoferric Oxide)
SB  - IM
MH  - Animals
MH  - Brain/*cytology
MH  - Cell Line
MH  - Contrast Media/pharmacokinetics
MH  - Dextrans
MH  - Female
MH  - Ferrosoferric Oxide
MH  - Iron/pharmacokinetics
MH  - Macrophages/*cytology
MH  - Magnetic Resonance Imaging/*methods
MH  - Magnetite Nanoparticles
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Confocal
MH  - Oxides/pharmacokinetics
MH  - Sensitivity and Specificity
EDAT- 2005/12/13 09:00
MHDA- 2006/03/24 09:00
CRDT- 2005/12/13 09:00
PHST- 2005/12/13 09:00 [pubmed]
PHST- 2006/03/24 09:00 [medline]
PHST- 2005/12/13 09:00 [entrez]
AID - 10.1002/mrm.20747 [doi]
PST - ppublish
SO  - Magn Reson Med. 2006 Jan;55(1):23-9. doi: 10.1002/mrm.20747.