PMID- 163658
OWN - NLM
STAT- MEDLINE
DCOM- 19750612
LR  - 20220408
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 45
IP  - 3
DP  - 1975 Mar
TI  - Human chronic myelogenous leukemia cell-line with positive Philadelphia 
      chromosome.
PG  - 321-34
AB  - A cell-line derived from a patient with chronic myelogenous leukemia (CML) is 
      described. The new cell-line, which has over 175 serial passanges in a 3 1/2-yr 
      period, has the following characteristics: (1) CML cells started to proliferate 
      actively since they were first incubated in culture media. A threefold increase 
      in the total number of cells was observed during the first seven passages; the 
      cell population increased by a factor of 10 to 20 every 7 days from passage 8 
      through 85; from 20 to 40 times from passage 86 through 150, and more than 40 
      times after 150 passages. (2) The majority of the nononucleated cells are 
      undifferentiated blasts. (3) The karyotype of all the cells examined show the 
      Philadelphia (Ph1) chromosome and a long acrocentric marker plus aneuploidy. The 
      Giemsa-banding studies identified the Ph1 chromosome as a terminal deletion of 
      the long arm of chromosome 22:del(22)(q12) and the long acrocentric marker as an 
      unbalanced reciprocal translocation of one chromosome 17 and the long arm of one 
      chromosome 15. (4) The CML cells do not produce immunoglobulins, are free of 
      mycoplasma, Epstein-Barr virus, and herpes-like virus particles. (5) CML cells 
      have no alkaline phosphatase and myeloperoxidase activities and did not engulf 
      inert particles. (6) Cultured CML cells provide a constant source of a specific 
      antigen. This CML cell-line represents a unique source of CML cells with 
      meaningful indicators of malignancy for clinical and experimental studies.
FAU - Lozzio, C B
AU  - Lozzio CB
FAU - Lozzio, B B
AU  - Lozzio BB
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Immunoglobulins)
RN  - 0 (Polystyrenes)
RN  - 10028-17-8 (Tritium)
RN  - EC 1.11.1.- (Peroxidases)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Alkaline Phosphatase/blood
MH  - *Cell Line
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, 21-22 and Y
MH  - Clone Cells
MH  - Culture Techniques
MH  - Female
MH  - Herpesvirus 4, Human/isolation & purification
MH  - Humans
MH  - Immunodiffusion
MH  - Immunoglobulins/analysis
MH  - Karyotyping
MH  - Leukemia, Myeloid/*genetics
MH  - Leukocytes/enzymology
MH  - Middle Aged
MH  - Mitosis
MH  - Mycoplasma/isolation & purification
MH  - Peroxidases/blood
MH  - Phagocytosis
MH  - Pleural Effusion/cytology
MH  - Polystyrenes
MH  - Thymidine/metabolism
MH  - Translocation, Genetic
MH  - Tritium
EDAT- 1975/03/01 00:00
MHDA- 1975/03/01 00:01
CRDT- 1975/03/01 00:00
PHST- 1975/03/01 00:00 [pubmed]
PHST- 1975/03/01 00:01 [medline]
PHST- 1975/03/01 00:00 [entrez]
AID - S0006-4971(20)66545-4 [pii]
PST - ppublish
SO  - Blood. 1975 Mar;45(3):321-34.